What is the most controllable (marine) bacterial culture you can cultivate in simulated seawater?

02 December 2020 6,796 3 View

Are there studies of the Effect of Social Media Memes on COVID 19 Information?

Dear Colleagues, I put up this question at RG in order to find out what is being studied about the effects on people of social media memes as they attempt to find reliable information regarding...

17 May 2020 1,398 18 View

Is there a program to pull only nouns or verbs from a text?

Dear Colleagues, I am compiling a list of words for an index and I wondered is this exists--sure it does somewhere. The index is for a collection of literary analysis essays. Thanks, Gloria

25 February 2020 1,755 4 View

How compare and order two dataframes in Rstudio?

Hello, I've done an Illumina 850k analysis and I received just the .idat files and a .rds file with bvalues already normalized. I would like to highlight the reference info and I've download the...

11 February 2020 5,900 2 View

Does 36B4 really is a unique copy gene?

02 September 2019 3,570 2 View

How long can the fresh Lentivirus store at 4 degree?

I make lentivirus weekly, but just use 30% of total volume. the leftover is aliquoted as 1 ml each tube then freeze at -80 freezer. Questions: 1. is this store method correct? directly put in -80...

08 April 2019 4,628 4 View

Is it possible for peroxide values of Ximenia oil to be affected by post harvest handling or length of storage ?

We measured peroxide values of Ximenia americana and X.cafra and the values were very low (3.7). i was just wondering if maybe post-harvest activities might affect this value.

14 May 2018 2,662 1 View

Can anyone help with theory on cultural transmission via science fiction, fantasy, and horror (gothic-based) literature?

Anthropologists generally study behaviors that persist in human groups. The invention of print media gave a new area for the preservation and transmission of cultural values. Literature,...

16 February 2018 6,397 7 View

Anyone working with Gardnerella vaginalis??

Good morning. Currently I am, working with G. vaginalis, but I have some problems to find an optimal medium to grow this bacteria. I used Casman Broth but the optical density of the culture was...

07 February 2018 4,211 2 View

Psychometric methods of testing the factor structure of a scale?

I want to examine the factor structure of two scales respectively (scale A: 14 items; scale B: 26 items). We are trying several methods: CFA followed by EFA; Rasch analysis and IRT respectively....

24 August 2017 2,254 8 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

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02 March 2021 3,060 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View