Gloria Marino

2 Questions 2 Answers 0 Followers

Questions related from Gloria Marino

What is an effective way to ensure cells don't stick to the sides of the tube after centrifuging when using PBS/EDTA to dissociate them?

For an experiment I am required to use a non-enzymatic dissociation buffer on my cells. I lifted 1205Lu melanoma cells off a T75 flask with 2mL of 5mM EDTA in DPBS. However, after centrifugation...

15 August 2018 7,918 2 View

Is there a 6-well plate for cell culture available with a more secure/locking lid?

We are looking for culture plates with lids that do not lift as easily outside the incubator. Alternatively, any techniques people have for how to avoid accidental lifting of plate lids would be...

02 August 2018 9,196 3 View