What make colistin toxic to kidney and nervous system?

thank you in advance chooseel

02 March 2018 2,373 1 View

Are there any website/textbook that make statistics easy to understand?

Most of textbook/website try to explain with too much detail (past few day I found textbook "statistic in a nutshell" but it's 400 pages ). some create a good diagram but explanation is not clear....

11 December 2017 7,477 1 View

What is the easy and cheap way to check size of total rna after total rna extraction for RNA-seq.

Thank you in advance

07 August 2016 3,328 3 View

Someone please explain about calculation of number of gap from Lander-Waterman model?

03 April 2016 5,490 2 View

Anyone can download Artemis software manual?

i don't know reason why I cannot download it from link below. note that I'm rookie in bioinformatics it would be nice if anyone attach the file thank you so much

01 February 2016 4,089 2 View

Anyone is familiar with 5% buffer-equilibrated phenol [pH7.4] in ethanol as stop solution before RNA extraction?

this reagent used to preserve mRNA, so it should be appropriate for my experiment for RT-qPCR and RNA-seq I spend time to find where original paper is but seem like no one cite the paper or just...

01 February 2016 2,453 9 View

MS Excel: can I run number in specific manner with specific digit sum?

31 December 2014 3,581 0 View

How do I know which hour to harvest bacterial cell for RNA extraction after exposed to antibiotic?

It's about transcriptomics. mid-log phase bacteria divided into 2 conditions, with and without antibiotic but I wanna know the incubation time before RNA extraction some paper said 30min, some...

11 December 2014 9,899 5 View

Could anyone order steps after receiving raw data from RNA-seq?

11 December 2014 2,811 3 View

What is the material inside the bench-top cooler?

10 November 2014 1,476 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View