Chooseel Bunsuwansakul

14 Questions 12 Answers 0 Followers

Questions related from Chooseel Bunsuwansakul

What make colistin toxic to kidney and nervous system?

thank you in advance chooseel

03 March 2018 2,494 1 View

Are there any website/textbook that make statistics easy to understand?

Most of textbook/website try to explain with too much detail (past few day I found textbook "statistic in a nutshell" but it's 400 pages ). some create a good diagram but explanation is not clear....

12 December 2017 7,573 1 View

What is the easy and cheap way to check size of total rna after total rna extraction for RNA-seq.

Thank you in advance

08 August 2016 3,420 3 View

Someone please explain about calculation of number of gap from Lander-Waterman model?

based on poisson distribution, probability of each nucleotide will be sequenced is P(Y=y)= (Cy)(e-c)/y! y=number of times base is sequenced C= coverage =NL/G = (#reads)(length of read)/genome...

04 April 2016 5,595 2 View

Anyone can download Artemis software manual?

i don't know reason why I cannot download it from link below. note that I'm rookie in bioinformatics it would be nice if anyone attach the file thank you so much

02 February 2016 4,186 2 View

Anyone is familiar with 5% buffer-equilibrated phenol [pH7.4] in ethanol as stop solution before RNA extraction?

this reagent used to preserve mRNA, so it should be appropriate for my experiment for RT-qPCR and RNA-seq I spend time to find where original paper is but seem like no one cite the paper or just...

02 February 2016 2,563 9 View

MS Excel: can I run number in specific manner with specific digit sum?

for example, sequence of 5 digits (only 1,2,4,6,9). It's should be 55 sequences right? like this... 11111 11121 11141 11112 11122 11142 11114 11124 ... 11116 11126 ... 11119 ...

01 January 2015 3,678 0 View

Is the reagent such as RNAprotect (Qiagen) really essential for bacteria?

Many bacterial RNA-seq research papers use it before RNA extraction. Is it critical that cells are harvested and then extracted immediately? Thank you in advance. (Newbie in RNA-seq.)

01 January 2015 2,166 1 View

Could anyone order steps after receiving raw data from RNA-seq?

I wanna see big picture and management of RNA-seq data like 1. quality of read: base calling, length of read,....... 2 screening: contamination, mismatch criteria,......... 3...... I have read...

12 December 2014 2,895 3 View

How do I know which hour to harvest bacterial cell for RNA extraction after exposed to antibiotic?

It's about transcriptomics. mid-log phase bacteria divided into 2 conditions, with and without antibiotic but I wanna know the incubation time before RNA extraction some paper said 30min, some...

12 December 2014 9,985 5 View

What is the material inside the bench-top cooler?

just wonder, Can I make DIY bench-top cooler? thank you in advance

11 November 2014 1,574 0 View

Are there any research papers or textbooks mentioning about how is importance to use log-phase bacteria?

I know that It depend on purpose of experiment but I wanna know the significance thank you in advance

11 November 2014 9,634 1 View

Is Optical Density value at midlog of bacterial growth curve the same as with the addition of an antibiotic?

Should the highest level of RNA expression should be on log phase? To compare the difference of RNA expression, can I use OD value of a cell at the midlog of bacterial growth curve to extract RNA...

10 October 2014 2,528 8 View

Why do we need to study mechanism of drug resistance in bacteria

good hygiene, drug of choice, and hospital management can decrease resistant bacteria so why I'm newbie ^ ^

10 October 2014 9,966 0 View