Is the quality of our RNA sufficient for RNA sequencing?

Hi, We are checking the integrity of RNA isolated (using MagMAX 96 Total RNA Isolation kit) from mice tissue on a 1% agarose gel that contains a bit of bleach to get rid of RNases. Our 28S/18S...

23 November 2020 5,006 3 View

How to dye the spider gland of a spider to see in fluorescence (488nm)?

I want to use a fluorescence microscope to observe in situ the venom gland of a cleared spider using a 488 nm laser. Any recommendation on what dye to use that would be particularly attached to...

14 October 2020 3,986 10 View

Book for demographic modelling?

Dear all, can anyone recommend a good introduction to demographic modelling in population genetics? I am mainly interested in books but paper suggestions are also welcome.Thanks a lot! Best...

10 June 2020 8,626 2 View

What is the best wall color for optical MoCap systems?

What is the best wall color of the lab to use with a IR cameras MoCap system (Qualisys, Vicon, Optitrack...)? We have always used white walls, without any problems. I read that blue color is the...

04 December 2018 7,946 2 View

Which is the impact of the education degree on the telecom industry profitability?

I need to know if there are studies about this: 1. Education degree of the telecom emplyees. 2. Age of the employees. 3. Eduactional degree type.

01 October 2017 1,999 3 View

What is the best option for a wearable metabolic system?

I would like to acquire a wereable metabolic system to use with a MoCap system in sports studies (jumping, running in a treadmill) and in human movment analysis studies (gait in a walkway,...

30 March 2017 3,036 4 View

What causes a failure of read error correction in Quorum (MASURCA)?

Dear all, I am currently trying to run the MASURCA assembler with my Illumina paired-end reads. The first steps work fine but when it comes to the error correction with Quorum the program reports...

13 July 2016 7,629 2 View

Extraction from bee museum specimens?

Dear all, I aim to amplify several nuclear genes (500-1100 bp) for dried museum specimens of the bee genus Rediviva (family: Melittidae). We got one middle leg per specimen for extraction...

29 June 2016 6,792 6 View

If I have cultured keratinocytes expressing only K14 gene, is it possible that under certain conditions, they become activated and express K16?

18 December 2014 1,476 6 View

3D imaging for biological surveys, collections or taxonomy research groups?

Hi. I'm a marine biologist close to finishing his phd in the use of 3D imaging technologies (CT Scan, microCT, laser triangulation, multi-image photogrammetry, focus stacking, light sheet...

07 August 2014 8,963 4 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

Which way will be better to give OGT inhibitors doses through intravenous or orally for pregnant female rats and concentration suggestion?

I'm planning to use ST078925 OGT inhibitor from TimTec (OGT-O-linked N-acetylglucosamine (GlcNAc) transferase) for female rats.

16 February 2021 7,850 2 View

Fluo 3-AM in pancreatic beta-cells?

Dear all, I'm trying to understand if a molecule of my interest (capable of inducing glucose-stimulated insulin secretion) determines an increase in intracellular calcium levels in the presence of...

16 February 2021 453 2 View

How can you tell if your organotypic slice culture is healthy ?

I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...

15 February 2021 2,830 2 View

Why daily dexamethasone given to rats fed high fat/fructose/salt cause very high mortality rate?

rats were given daily dexamethasone in drinking water corresponding to 0.5 mg/kg or 2 mg/kg. These rats are simultaneously fed 25% fat, 3% salt in diet and 10% fructose in drinking water but...

15 February 2021 7,634 2 View

How to calculate the sample size required to detect the difference in pharmacokinetics of methotrexate?

I came across with this study here, https://www.clinexprheumatol.org/abstract.asp?a=5066 And this study says: "The sample size calculation was done to compare two different MTX starting dose...

14 February 2021 3,017 3 View