17 December 2014 1 297 Report

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How can i generate a CRISPR knockin mutation zebrafish model with a reporter?

Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...

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Is grammaticalization/subjectification a mechanism or motivation?

I know that we can categorize a specific linguistic change as either gramaticalization or subjectification (or both involved), but can we also say that specific linguistic change is motivated by...

31 May 2024 2,106 2 View

Could someone help a right procedure for TGA analysis of cement?

I analyzed cement samples exposed and unexposed to moisture over various periods. The TGA results of these samples showed variation with each repetition. However, I consistently used calcium...

22 May 2024 1,339 3 View

How many brain slices do we need to obtain 1 mg of protein for biochemical studies?

We are currently interested in performing a stimulation protocol on brain slices in order to perform western blotting or immunoprecipitation. However, we do not know how many 300 um slices would...

12 May 2024 6,527 1 View

Are you interested in W. Somerset Maugham?

We are organising a two-day international interdisciplinary conference on Maugham's life and works, in Le Mans, France on 13-14 March 2025. If you are interested, please read our CFP and send us...

12 May 2024 5,473 2 View

Does anyone have experience interpreting the output generated by Bottleneck software?

We are investigating the genetic structure of a population. We utilized the software 'Bottleneck.' Does anyone have experience interpreting the output generated by Bottleneck software?

17 April 2024 4,388 0 View

Can you identify what is this in my cell culture?

Hi, I have been cultivating Jurkat cells in RPMI supplemented with 10% FBS, and I have observed some small things in my flask. Although the cell growth appears unaffected, I want to ensure that...

16 April 2024 5,466 3 View

Solution Chloroplatinic acid?

What volume of Chloroplatinic acid is required to form 10 ml of a solution in which it is 16 mM? The density of Chloroplatinic acid is 2.43 g ml-1, and its molecular weight is 409.81. I really...

18 February 2024 7,908 2 View

Can i publish articles in reputed journals related to teachers as i am already working as an Assitant Profesor?

I am doing Ph.D studies on Organisational Citizenship Behaviour of University Teachers. I am also working as an Assistant Professor. I want to publish articles on OCB relating to Teachers. Can i...

24 January 2024 4,135 3 View

How to calculate DBH for trees with multiple dead and live stems in the same tree?

I am trying to calculate DBH (diameter at breast height) in order to determine the demography and basal area of trees. However, some of the trees have multiple stems which makes it difficult to...

23 January 2024 845 0 View

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Can I use a HisTRAP column for affinity chromatography?

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Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

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Weak DAPI staining after immunohistochemistry - how to improve?

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Are the GABAergic neurons electrophysiologically equal in all brain areas?

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Why results of ROS flurescence are negative as there was no bacteria within?

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