Can anyone provide me a protocl of Native PAGE with the 30% terminal resolving gel concentraion?

I am separating the AuCNs, which are Au nanoclusters. I used the 3% stacking and 30% resolving polyacrylamide from the reference. Eachtime when the resolving gel is polymerizing, plenty of heat...

22 January 2021 8,522 3 View

Naming the "UK coronavirus variant" rises to the occasion?

In the context of the COVID-19 infodemic, the global profusion of monikers and hashtags for "UK coronavirus variant" have found their way into daily communication and contributed to a backlash...

01 January 2021 7,850 2 View

Conceptual metaphor: will multiple target concepts be activated once a single source domain is activated?

It is common that a source domain is conceptually linked to multiple target domains. According to the neural theory of metaphor, once a concept as a source domain is activated, signals will spread...

28 September 2020 6,106 4 View

Number of Estimating Equations Required to Test Hypotheses?

After literature review, current draft as below: Variables: Independent = B (represented by I, M, O, S, C), Moderating = W Dependent = F Control = CV Hypotheses: H1a. I has significant...

18 September 2020 7,055 2 View

Why is my protein a smear when I detect with CoIP lysis buffer plus LDS?

Hi I am trying to express my protein R in tobacco leaves and do a CoIP, but I can't detect the protein R in CoIP input. I took the same tissue and added 8M Urea plus LDS protein buffer, protein R...

31 August 2020 9,534 10 View

What's the main difference between #Tris-glycine #SDS-PAGE and #Bis-Tris #SDS-PAGE. How pH and ion strenght affect SDS-PAGE?

sometimes, there is some smear on the SDS-PAGE, this can be eliminated with a different running buffer or a different PAGE, or a different way of sample loading buffer. how these affect the SDS-PAGE?

09 August 2020 6,149 3 View

Is there a gentle method to reduce cuprous oxide to copper while maintaining the original morphology?

Hello, Recently, I have synthesized some cuprous oxide nanomaterials and want to reduce them to metal while maintaining the morphology. Is there a way to do this?

04 August 2020 4,850 3 View

Is there a function coded by matlab to reproject the geographic raster data?

Is there a function coded by matlab to reproject the geographic raster data?especially for the case converting the Ease-Grid 2.0 to the geographic coordinate.

17 June 2020 7,875 2 View

Why the contaminated colony grow around the plate?

Doing the antibacterial test, the unknown colony grows around the plate. E. coli and antibacterial regent were mixed with LB, PBS and EDTA. Not likely the contamination was from the LB, PBS or...

18 May 2020 1,311 3 View

How to do a two-way ONOVA in SPSS?

I don't have advanced knowledge of SPSS and tutorials aren't making much sense to me, if anyone is able to explain SPSS two-way ONOVA step-by-step in the simplest form I would be grateful. My...

30 March 2020 8,769 2 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

No title

02 March 2021 3,060 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

No title

28 February 2021 9,936 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View