I am trying to reprogram MEF and HDF into iPSCs using trasfection reagent like X-trem Gene, Lipofactamine2000 and Lipofactamine3000 but the transfection efficiency is low. I also have NEON transfection system but I was just wondering that is it safe for cells to re-transfect several times using electroporator?
As reprogramming is a very sensitive process and during electroporation we have to detach and re-attach cells which might disturb the reprogramming process or even survival of the cells. Does anyone has experience to do such kind of reprogramming experiment or atleast successful re-electroporation of same cells? Please enlighten me with your experience. Thank you!
Did you ever tried the RNAimax as transfection agent?
I'm asking because I did and get 90% of efficiency in my case, but it is another type of adherent cells.
Dear Pedro,
I didn't use RNAimax but tried lipofactamine2000 which didn't work in case of my primary cell line (nHDF). So, I want some opinion about safety of re-transfection using electroporation. Do you have some experience?
Sorry, I have experience only with lipofectamine2000 and RNAimax.
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