Hi Everybody. I'm senior in a University in Vietnam. This is fisrt time I do SDS-PAGE, so I want to ask about the correction of my loading buffer recipe for SDS-PAGE as a following file.
In addition, If 0.5M Tris-HCl pH 6.8 isn't available, Can I replace it by 1M Tris-HCl pH 6.8 or any other concentrations of Tris-HCl pH 6.8.
Also, how should I mix these components? (In order or out of order)
(Samples I am using in my research is piglet intestines)
The recipe looks good.
Of course you can use 1M Tris/HCl (or any other concentration), just adjust the volume and add appropriate amount of water.
The order of addition doesn't matter, but I suggest adding the mercaptoethanol last, and working with it in a fume hood, if one is available, because it is very smelly. Your lab mates will not be pleased with you if you open the stock bottle out in the open air.
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