Is it true that the toxic effects of a lead are more severe to the sensory nerve compared to the peripheral nerve?

More Shamsul Bahari Shamsudin's questions See All

DNA Extraction from old clot blood?

I want to separate DNA from human old clotted blood . I haven't any idea how to extract DNA from it. Your kind expertise and thought would be appreciated.

07 October 2020 7,871 2 View

How to calculate linear refractive index value from the uv-vis spectrum for the liquid sample?

is there any specific equation to calculate the linear refractive index at different wavelengths of the liquid sample from the UV-Vis spectrum? thank you

22 September 2020 5,839 4 View

What is the latest trend or updates in the study of Gamification in education, especially in enhancing engagement and motivation?

Seeking the latest development in gamification research particularly in education whereby gamification is used to increase student engagement with the lessons and later improve their motivation to...

07 October 2019 2,743 2 View

How to check secretory protein concentration from cell culture media by spectrophotometer?

I am going to culture a cell line with 20% FBS in IMDM medium. Those cell will secrete protein. I need to check the protein concentration from the culture medium by using spectrophotometer. Can...

17 September 2019 6,047 1 View

How to prepare bacterial complex of several different strain of bacterial mixture to treat mouse.??

I want to treat a bacterial mixture to mice. I have 6 different strain, now how should I prepare the bacterial complex mixture to mouse.

03 June 2018 8,575 2 View

Quercetin, How much I should administrate to mice?

For quercetin how much I should administrate to mice, and what will be the administration route? Please suggest

16 May 2016 3,454 2 View

What titrant should be use in titration of sample in DMSO?

I am doing titration of sucrose stearate in DMSO solution. I had tried using ethanolic KOH and NAOH but when i titrate the control, the volume of titrant does not follow the theory volume. So,...

14 January 2016 5,511 3 View

When I add 1 mM B mercaptoethanol with 1 mM KAuCl4, a white precipitation occurs straight away. Is that the thiolate-protected gold clusters ?

Need some help on Gold Chemistry !!

29 November 2015 6,585 3 View

What is the most accurate biological sample that can indicate heavy metals exposure?

Human biological sample...

09 February 2015 3,420 21 View

What is the meaning of my Theta-2 theta scan in XRD measurements for Heteroepitaxial growth of GaAs thin films on silicon?

To evaluate the material quality, I have measured the XRD of my as-grown structures by performing theta-2theta (TTH) scan which exhibits poly-crystallinity as shown in the attached file. I have...

04 August 2014 2,679 53 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

How to dilute Toxin-DMSO solution?

I have a concentrated solution of a toxin(50 micromolar) in DMSO 100%. I need to dilute it inorder to dilute both the toxin and DMSO wich can be toxic to cells. Which would you guys recommend to...

19 February 2021 7,447 2 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

What lysis buffer is compatible with Bradford assay?

Hello! I am planning to use Bradford assay to measure protein content in PBMCs lysates. The problem is that Bradford assay doesn't seem to be compatible with Tris-based buffers or SDS. Are there...

10 February 2021 1,797 5 View

Macrophages in culture not growing/ dying or detaching . Why ?

Hello, I am an undergraduate student doing my dissertation, where I isolate PBMCs from leucocyte cones in order to plate monocytes and allow for macrophage differentiation. Although the numbers...

09 February 2021 5,334 8 View

Has anybody administered MK-2206 (an orally active, allosteric inhibitor of AKT) intravenously to the mice?

We aim to use MK-2206 to inhibit AKT in mice. However, a large body of studies suggests that it should be administered orally. I was wondering if somebody could tell me that is it toxic for the...

07 February 2021 6,340 1 View