Is it possible to find IC50 in Graph Pad Prism from cell viability data?

Nur Farisya Shamsudin @Nur-Farisya-Shamsudin

13 June 2022 1 5K Report

Hello. I am a beginner at using Graph Pad Prism software. I want to ask, is it possible for me to find the IC50 value for the cytotoxicity test if I only have the data for cell viability (%)?

Adem Özleyen

Dear Farisya,

Graphpad Prism is a very useful tool to calculate the IC50 values of the cytotoxicity, enzyme inhibition, ELISA based assays. It also provides good quality of figures for potential publications.

This video will help you to manage your data for the calculation.

https://www.youtube.com/watch?v=7NgRqXSByFo

Bests

Adem

Badges
Science topic

Similar topics
Phytochemistry
Extracts

More Nur Farisya Shamsudin's questions See All

Is there a subtle distinction between the terms "substitution" and "doping"?

Dear Prof, is there a subtle distinction between the terms "substitution" and "doping" when referring to modifying ceramic dielectric materials for example Na0.5Bi0.5TiO3? Is there any difference...

08 July 2024 2,043 3 View

Problems/ Challenges regarding Dark Tourism?

Im currently writing my Thesis on Challenges/ Problems/ Particularities regarding Dark Tourism and have a little trouble finding the right literature. I have found a lot of literature for dark...

14 June 2024 7,262 2 View

When IC50 cannot be obtained according to the cell viability chart, what should be the dose selection under cell culture conditions?

When IC50 cannot be obtained according to the cell viability chart, what should be the dose selection under cell culture conditions? Would it be beneficial to go with sham control? For example,...

11 June 2024 9,109 0 View

How to calculate my FRAP Assay?

Y=0.0011x+0.1031 (Standard curve eq) Chamomile sample 20mg/mL Absorbance(y)= 1.16 x=960.82(µg/mL) Chamomile sample 10mg/mL Absorbance(y)= 0.80 x=633.55(µg/mL) Tridax sample...

01 June 2024 7,843 0 View

What are the long-term functional and aesthetic outcomes of autologous vs alloplastic materials in facial reconstructive surgery following trauma?

This question aims to explore the comparative effectiveness of using a patient's own tissue (autologous) versus synthetic materials (alloplastic) in facial reconstructive procedures, focusing on...

25 May 2024 1,791 0 View

How to determine the concentration of MXene colloidal solution?

Dear researchers, I have recently synthesised MXene by etching MAX phase source using in-situ fluoride based etchant and collected the supernatant solution obtained after the washing process. My...

22 May 2024 5,269 2 View

Do your country have Psychology Acts?

Hi/Salam; Did your country establish "Psychology Acts" for psychologists to practice? I just discovered that Malaysia still does not have the Psychology Acts for psychologists to practice, where...

13 May 2024 6,921 6 View

Dear Prof and Dr, how to calculate the recoverable energy density from P-E hysterisis loop ?

The recoverable energy density can be evaluated by the following equation: Wrec = ∫P EdP = Area CDBCJcm−3 (4) Thus, when domains are realigned by the applied electric field, the difference...

08 May 2024 4,561 0 View

RMSD plot from GROMACS output file .xtc did not start from 0ns. How to get the 0ns frame?

Dear GROMACS expert, I am currently having trouble to analyse the results from my simulations. After processing the output files using this command, gmx trjconv -s md_0_1.tpr -f md_0_1.xtc -o...

02 May 2024 6,690 7 View

How can I get involved in writing a chapter of scientific book in my research area?

Any lead will be helpful

24 April 2024 5,987 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How can I calculate IC50 in DPPH test by using graph pad prism software?

How can I calculate IC50 in the DPPH test using graph pad prism software? or suggest a manual method and other software to calculate the IC50 value.

06 August 2024 8,848 3 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View