How to prepare bacterial complex of several different strain of bacterial mixture to treat mouse.??

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Valerie Diane Valeriano

Hi, a few things to consider. Are your bacterial strains aerobic or anaerobic? How do you plan to administer the bacterial mixture? At what CFU/bacteria is your target bacterial concentration inoculation?

Maybe this journal can also be of help in planning your preparation. http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1001637#pbio.1001637.s011

Jay Siddharth

https://www.taconic.com/prepare-your-model/microbiome-solutions-and-germ-free-mice/protocols-colonizing-axenic-rodents.html

This a protocol for ASF mice..similar to what you might be attempting to do.