Can anybody provide me sequences of SPF10 primers?

03 February 2021 1,053 2 View

How to input an exogenous variable in a GARCH model?

Hello, I have a question regarding financial time series. I am studying the effects of Quantitative Easing on the US stock market. I have tried to fit an ARIMA model to my S&P500 returns with...

01 February 2021 8,325 3 View

Kruskal-Wallis Test does not offer results in Model Viewer (SPSS 26.0)?

One of my students faces this problem in SPSS 26.0: When he runs Kruskal-Wallis test, the Output does not offer him access to the Model Viewer (where the pairwise comparison etc. would be...

11 November 2020 6,345 8 View

Can you recommend suitable primary antibody for Tau detection in rat?

Hi, I have problem detecting tau in rat primary astrocyte lysate by WB. I have used Tau46 form Santa Cruz Biotech (Sc-32274) but I don't see any band not even in positive control (rat brain...

23 May 2019 4,564 3 View

How to model utility grid in solar grid tied system in Simulink?

I am modelling grid tied solar PV with backup battery and I inserted load between solar system and grid. According to the graph of rms currents: Igrid = Ipv + Iload, which doesnt make any sense to...

10 May 2018 4,694 2 View

SPSS error "subscript is out of range", what to do?

21 January 2018 9,637 4 View

How to explain different patterns resulting after acridine orange assay for assessment of sperm DNA integrity?

We have performed AO assay on human ejaculated sperm. Everything seems, that works well, however we observed und microscope every cells stainned green and moreover there are two distinct...

02 October 2017 3,273 3 View

Method to make thin layer on glass substrate from nanopowder scintillator

Hi, does anyone have experience with making a thin film from scintillator nanopowder or nanopowder in general (GGAG:Ce, LuAG:Ce ... 10 - 30 nm particle size) on glass substrate. I writing master's...

14 August 2017 1,964 1 View

Does anyone experience with sonication of HCT116 on Bioruptor Pico?

17 July 2017 9,180 1 View

How do I decrease protein expression from pETDuet vector?

08 February 2015 7,968 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View