Can be used RNase inhibitor which is normally used for isolation of RNAs to RNAse treatment in blood plasma?
Could you please elaborate the question. what do you mean by blood plasma here (isolated)?? and which organism's plasma is it???
Hello! I would need to orientate myself in one aspect of the industrial production of therapeutic monoclonal antibodies. I understand the technologies used for their development (hybrioma, phage...
13 March 2024 1,200 2 View
Charlie
10 December 2023 1,615 1 View
Hello everyone, I am having technical difficulties with entering the website of AraENCODE from the link (http://glab.hzau.edu.cn/AraENCODE/) from the original article - AraENCODE: A comprehensive...
11 October 2023 1,956 3 View
I performed a DSC analysis of a 7075 aluminum alloy during which the sample was melted. After analysis, the whole sensor was covered with a thin metal layer (probably mainly zinc and aluminum). It...
03 November 2021 1,420 8 View
Can traces of Ficoll impact absorbance A260/230 during RNA extraction? Basically, does Ficoll absorb at 230 nm? We can't find this information as we blame Ficoll for the low A260/230 in some of...
24 October 2021 2,419 4 View
Dear scientists, currently I'm doing research on how skid trails (or forest roads) affect hydrology of forest basins. More precisely I'm looking for studies that tried to found out if there is...
23 September 2021 646 0 View
Hi, I´m running PCR testing of HPV in cervical smears. At the moment I´m using BS-GP5+/6+ primers (Schmitt. et al. 2008), but I would also like to test SPF10 primers, because I have some problems...
03 February 2021 1,383 2 View
Hello, I have a question regarding financial time series. I am studying the effects of Quantitative Easing on the US stock market. I have tried to fit an ARIMA model to my S&P500 returns with...
01 February 2021 8,595 3 View
Hello, I am working on LCMS method for steroid hormones and I see a big difference between untreated serum and steroid-free charcole stripped serum. Raw extracts are different, there are almost no...
20 December 2020 1,110 1 View
One of my students faces this problem in SPSS 26.0: When he runs Kruskal-Wallis test, the Output does not offer him access to the Model Viewer (where the pairwise comparison etc. would be...
11 November 2020 6,734 8 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View