11 Questions 23 Answers 0 Followers
Questions related from Keerthi Bandara Jayasundera
In my LC-MS experiment, I am utilizing an Agilent Polaris NH2 column to separate a mixture of glucose, fructose, and sucrose. I am using water and acetonitrile as A and B mobile phases...
27 March 2024 8,856 4 View
I am using a C30 column for carotenoid analysis. I bought the column recently and using it for the first time. When I use the column in LC-MS scan mode, I clearly see the UV-Vis peaks for my...
09 June 2023 2,319 2 View
I am analyzing Lycopene using LC-MS. I use a C18 column and my mobile phase is Methanol: MTBE = 95:5. I use an Agilent Ultivo QQQ mass spectrometer with APCI negative ion mode. I see the precursor...
08 May 2023 487 0 View
I am running an HMBC experiment on a Bruker 300 mHz instrument. I set up the experiment following the steps given in TopSpin guide book. Everything happens fine but when I try to open the spectrum...
10 March 2023 5,261 4 View
I need to concentrate some fatty acid methyl esters in a cell culture medium to separate and isolate them using Prep-HPLC. What type of solid phase extraction would be suitable to concentrate...
03 March 2023 9,484 0 View
I used the following column for an HPLC separation; C18 4.6 x 250 mm, 5 um, 120 Ao. I used methanol and acetonitrile as mobile phases. Now I have to do LC-MS for the same samples. I am wondering...
20 December 2022 1,865 6 View
I want to know some cheap, easily available compounds that can be used as substrates to detect laccase activity instead of ABTS using UV-Vis spectrometer.
28 April 2021 223 3 View
I have seen that in CNT -polymer composites the bonding strength (van der waals, covalent etc) has been predicted by simulation. Is there any method to experimentally measure bonding strength or...
22 November 2017 9,521 3 View
For some adsorption experiment we are using 10 ppm Pb(ii) solution. However Pb(ii) adsorb onto the filter paper significantly making it difficult to get a reliable reading from AAS. Does anybody...
18 October 2017 2,161 3 View
What would be a suitable vacuum pump for a solid phase extraction manifold (VAC ELUT SPS 24, 10 X 75MM RACK (from Agilent).
15 February 2017 8,120 5 View
I need to connect an -OH group to another molecule in one or two steps without harsh reaction conditions, easiest way seems like making an ester. But I think esters get hydrolyzed easily. Are...
23 October 2015 6,042 6 View