Is it possible to use produce a ESR1 shRNA cell line with MCF7s or T47D's without causing growth arrest? Has anyone done this? I could not find a successful reference in the literature. Many Thanks
Hi, Alice,I think you maybe need a drug-induced expression system to express ESR1 shRNA in cell lines. I suggest the tet-on gene expression system. The cell lines wont express ESR1 shRNA until you add Dox in the medium, that means the cell growth wont be arrested if you dont add Dox.