Anybody tried thisbefore? Pls let me know about the exact numbers of cells to be injected, route of injection, and duration to develop the cancer. TQ
We make HT-29 xenografts pretty regularly for preclinical drug evaluations (see http://altogenlabs.com/xenograft-models/colon-cancer-xenograft/ht29-xenograft-model/). We usually add a million cells in matrigel to the inoculation site (whether subcutaneous or orthotopic) and it takes around a month to get to sufficient tumor size (around 100 cubic millimiters) and then that's when we begin drug evaluations.
I was just reading a paper where they used the HT-29 for xenograft studies: Clin Cancer Res. 2012 June 1; 18(11): 3100–3111.
They injected 10 million cells (in 200 ul serum-free medium/ Matrigel 1:1) subcutaneous in the flank and had good tumour formation within a couple of weeks.
Dear Mr. Muller; many many thanks for your informative reply. Trying my best to work with it soon. TQ
Hi If you want on site colon tumour, using HT29, the cells must be injected orthotopically, i.e. into the colon tissue. This involves surgery and to monitor the growth, would be by imaging and immunoflourescence techniques. If you just want to study the growth rate then subcutaneous injection is fine, on the flank of the mouse. Please feel fre to contact myself, for any further detailed information.
I was not able to establish xenograft tumor growth by injecting 10^7 HT29 cells in 100mcl PBS subcutanously in CD1/nu nude mice. However, it worked nicely with Colo320 cells. I hope this helps. Good luck
I found HT29 cells had good growth curves when injected subcutaneously in Balb C nude mice (female) at a range of concentrations 2^6-1^7. Matrigel was also tested at the same time but was not necessary to get good growth in 2-4 weeks. Orthotopic models do exist but you'd need to buy a colonoscope etc - see this paper for info/videos: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0028858
Feel free to contact me if you want any more information on the subcutaneous model.
HT29 administered as suspension of 2.5x 105 HT-29 cells in 100 ul PBS s.c. into the flanks of nude mice are growing well in our laboratory (Kucerova L et al. 2007)
We make HT-29 xenografts pretty regularly for preclinical drug evaluations (see http://altogenlabs.com/xenograft-models/colon-cancer-xenograft/ht29-xenograft-model/). We usually add a million cells in matrigel to the inoculation site (whether subcutaneous or orthotopic) and it takes around a month to get to sufficient tumor size (around 100 cubic millimiters) and then that's when we begin drug evaluations.
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