I am looking for a tissue lysis buffer for immunophenotyping. TQ
Publication in both groups of journals are should not be used for academic promotion.
11 December 2019 5,373 8 View
I have run MTT for crude extract, pure compound and essential oil at the same time. I have used same batch of the cell, same number (0.8X 10,0000 cells/ml), same technique and at the same day, but...
06 July 2018 5,339 2 View
Some of my submitted manuscripts (5) got rejected by the journals, and want to submit into another journal, but I do not have enough time to format. If any one can provide me with the soft ware...
31 December 2017 2,905 36 View
In recent years several new mouse models for lung cancer have been described. These include models for both non-small-cell lung cancer (NSCLC) and small-cell lung cancer (SCLC). Tumorigenesis in...
11 December 2015 8,001 4 View
But like other treatments, it often causes side effects. These are different for each person. They depend on the type of cancer, location, drugs and dose, and your general health. How long the...
10 November 2015 7,730 3 View
Polymerase Chain Reaction is widely held as one of the most important inventions of the 20th century in molecular biology. Small amounts of the genetic material can now be amplified to be able to...
11 December 2014 6,353 47 View
I already used EndNote during my PhD study, but now want to learn more soft wares. I will be appreciable if any one help me in this issue. TQ
10 November 2014 5,511 10 View
Can anyone guide and help me to promote the growth of human melanoma cell line (SK-MEL-2) and murine melanoma cell line (B16). We bought both of them directly from ATCC 2 months ago. At begining...
06 July 2014 3,069 12 View
When we want to screen or test the effect of a natural product in cancer cells, we have to know how safe is it. Why do some articles mention antiproliferative and others cytotoxicity assays for...
05 June 2014 2,944 20 View
The Institute for Scientific Information (ISI) was founded by Eugene Garfield in 1960. It was acquired by Thomson Scientific & Healthcare in 1992, became known as Thomson ISI and now is part of...
02 March 2014 5,640 4 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...
03 March 2021 6,299 3 View
i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity
03 March 2021 8,890 1 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.
01 March 2021 9,952 3 View
I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...
01 March 2021 9,015 2 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...
27 February 2021 5,028 2 View