I am looking for a tissue lysis buffer for immunophenotyping. TQ
Publication in both groups of journals are should not be used for academic promotion.
11 December 2019 5,531 8 View
I have run MTT for crude extract, pure compound and essential oil at the same time. I have used same batch of the cell, same number (0.8X 10,0000 cells/ml), same technique and at the same day, but...
06 July 2018 5,503 2 View
Some of my submitted manuscripts (5) got rejected by the journals, and want to submit into another journal, but I do not have enough time to format. If any one can provide me with the soft ware...
31 December 2017 3,055 36 View
In recent years several new mouse models for lung cancer have been described. These include models for both non-small-cell lung cancer (NSCLC) and small-cell lung cancer (SCLC). Tumorigenesis in...
11 December 2015 8,196 4 View
But like other treatments, it often causes side effects. These are different for each person. They depend on the type of cancer, location, drugs and dose, and your general health. How long the...
10 November 2015 7,906 3 View
Polymerase Chain Reaction is widely held as one of the most important inventions of the 20th century in molecular biology. Small amounts of the genetic material can now be amplified to be able to...
11 December 2014 6,526 47 View
I already used EndNote during my PhD study, but now want to learn more soft wares. I will be appreciable if any one help me in this issue. TQ
10 November 2014 5,656 10 View
Can anyone guide and help me to promote the growth of human melanoma cell line (SK-MEL-2) and murine melanoma cell line (B16). We bought both of them directly from ATCC 2 months ago. At begining...
06 July 2014 3,241 12 View
When we want to screen or test the effect of a natural product in cancer cells, we have to know how safe is it. Why do some articles mention antiproliferative and others cytotoxicity assays for...
05 June 2014 3,280 20 View
The Institute for Scientific Information (ISI) was founded by Eugene Garfield in 1960. It was acquired by Thomson Scientific & Healthcare in 1992, became known as Thomson ISI and now is part of...
02 March 2014 5,819 4 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...
31 July 2024 5,645 2 View
I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...
29 July 2024 3,030 2 View