How to calculate response rate if the number of eligible respondents is not available?

Hi All, I am carrying out an international research with clinicians (speech-language pathologists/ therapists). International professional associations will be distributing the questionnaire link...

02 February 2021 5,306 3 View

How to report data about studies awaiting classification in a Systematic Review?

Hi All, I am currently undertaking a systematic review. 19 studies were classified as studies awaiting classification. I have contacted the authors. Several authors did not reply and a number...

22 July 2019 3,987 1 View

How should I compare sEMG data across different clinical groups?

Hi All, I am new to the field of sEMG so any guidance or feedback would be greatly appreciated. I am collecting facial EMG data from two groups: healthy vs individuals with neurological impaired...

19 June 2019 6,067 3 View

How should similar studies by the same authors be assessed in a systematic review?

Hi All, I am currently undertaking a systematic review. Two of the studies, completed by nearly the same authors, seem to have used the same set of participants. The differences mainly lies in...

30 May 2019 7,017 8 View

Is there any robust assay to quantify the maturation state of autophagosomes in vertebrates?

I am looking for a way to quantify autophagosome maturation before fusion with lysosomes to see if there is any defect in cargo recognition. I've read about co-localization studies of p62 and LC3...

26 May 2019 2,200 1 View

AMBER parameters for Boron (in BDPY-linked-ligand)?

Boron atom type is not present neither in GAFF neither in AMBER force field. Is there any ff implementation to parameter Boron? Do you have any suggestions to solve out the problem?

15 January 2019 7,264 10 View

How can I optimize a PCR of a 5 kb fragment using Q5 polymerase?

I have amplified this 5 kb fragment using LongAmp Taq DNA polymerase from genomic DNA, then I extracted the amplified product from the agarose gel and I have purified it. I have tried amplifying...

20 April 2017 1,380 2 View

What statistical test to use?

16 December 2015 8,657 12 View

How do I control genetic maps quality?

I have a map of a plant BC1 population i have drawn with MAPDISTO software and re-drawn with joinmap4.1 software. Both maps show linkage between two arabidopsis chromosomes probably due to...

01 October 2015 4,470 5 View

I have a soil sample and I want to distinguish how much of bacteria, fungi and plants are present. Any suggestions?

I need a precise detection technique to detect bacteria, fungi, plants in soil.

22 September 2013 6,979 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View