I tried different protocols but at the beginning of the procedure the tissue (starfish gonads) gelifies and blocks the other extraction phases.
Penso che possa saperne qualcosa il Professor Luciano Periale fisico del CERN di Torino che lavora all'UNI Di Torino. Una volta si occupava di codici genetici nei segnali magnetici che arrivavano in fondo all'oceano.
I need the contacts of a company able to furnish or support the set up of a RUSITEC, a continuous cell rumen model. Could do you be able in supporting me? Or, at least, do you have a project of...
25 January 2022 6,115 4 View
I have been asked to use the 284.8eV peak in the C1s narrow scan as the reference peak for fitting all other potential peaks for a carbon material. Also, which fitting optimization parameters...
11 January 2022 6,308 5 View
Hi All, I am carrying out an international research with clinicians (speech-language pathologists/ therapists). International professional associations will be distributing the questionnaire link...
02 February 2021 5,577 3 View
About 10 years ago, I experienced that the black leaf clips used with Pocket-PEA fluorometer (firmly suggested by Hansatech vs. the white version used for Handy-PEA), when used under high...
31 July 2020 5,853 0 View
I have some problem with baseline of my DAD (G1315B Agilent Technologies). I have tried to wash the cell (bypassing column) with isopropanol for a night. The issue seemed to be solved, but during...
10 December 2019 647 0 View
Hi All, I am currently undertaking a systematic review. 19 studies were classified as studies awaiting classification. I have contacted the authors. Several authors did not reply and a number...
22 July 2019 4,213 1 View
Hi All, I am new to the field of sEMG so any guidance or feedback would be greatly appreciated. I am collecting facial EMG data from two groups: healthy vs individuals with neurological impaired...
19 June 2019 6,178 3 View
Hi All, I am currently undertaking a systematic review. Two of the studies, completed by nearly the same authors, seem to have used the same set of participants. The differences mainly lies in...
30 May 2019 7,209 8 View
I am looking for a way to quantify autophagosome maturation before fusion with lysosomes to see if there is any defect in cargo recognition. I've read about co-localization studies of p62 and LC3...
26 May 2019 2,503 1 View
Boron atom type is not present neither in GAFF neither in AMBER force field. Is there any ff implementation to parameter Boron? Do you have any suggestions to solve out the problem?
15 January 2019 7,466 10 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...
31 July 2024 5,645 2 View
I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...
29 July 2024 3,030 2 View
My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...
29 July 2024 2,682 6 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
This is a slice of intestinal tissue from the Chinese mitten crab (intestine in the upper right corner). I injected WSSV into this horseshoe crab. Now I want to know what these red spots next to...
18 July 2024 5,602 0 View
The burden of obesity is enormous. The greater challenge lies in accurately diagnosing obesity at the right time, reflecting the true increase in visceral fat. BMI may not accurately represent...
14 July 2024 8,771 4 View