I tried different protocols but at the beginning of the procedure the tissue (starfish gonads) gelifies and blocks the other extraction phases.
Penso che possa saperne qualcosa il Professor Luciano Periale fisico del CERN di Torino che lavora all'UNI Di Torino. Una volta si occupava di codici genetici nei segnali magnetici che arrivavano in fondo all'oceano.
Hi All, I am carrying out an international research with clinicians (speech-language pathologists/ therapists). International professional associations will be distributing the questionnaire link...
02 February 2021 5,306 3 View
Hi All, I am currently undertaking a systematic review. 19 studies were classified as studies awaiting classification. I have contacted the authors. Several authors did not reply and a number...
22 July 2019 3,987 1 View
Hi All, I am new to the field of sEMG so any guidance or feedback would be greatly appreciated. I am collecting facial EMG data from two groups: healthy vs individuals with neurological impaired...
19 June 2019 6,067 3 View
Hi All, I am currently undertaking a systematic review. Two of the studies, completed by nearly the same authors, seem to have used the same set of participants. The differences mainly lies in...
30 May 2019 7,017 8 View
I am looking for a way to quantify autophagosome maturation before fusion with lysosomes to see if there is any defect in cargo recognition. I've read about co-localization studies of p62 and LC3...
26 May 2019 2,200 1 View
Boron atom type is not present neither in GAFF neither in AMBER force field. Is there any ff implementation to parameter Boron? Do you have any suggestions to solve out the problem?
15 January 2019 7,264 10 View
I have amplified this 5 kb fragment using LongAmp Taq DNA polymerase from genomic DNA, then I extracted the amplified product from the agarose gel and I have purified it. I have tried amplifying...
20 April 2017 1,380 2 View
16 December 2015 8,657 12 View
I have a map of a plant BC1 population i have drawn with MAPDISTO software and re-drawn with joinmap4.1 software. Both maps show linkage between two arabidopsis chromosomes probably due to...
01 October 2015 4,470 5 View
I need a precise detection technique to detect bacteria, fungi, plants in soil.
22 September 2013 6,979 2 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity
03 March 2021 8,890 1 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...
27 February 2021 5,028 2 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...
24 February 2021 9,210 1 View
I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...
24 February 2021 3,623 3 View
22 February 2021 9,932 11 View
Our team has been trying to locate and collect the pineal gland in the American bullfrog's brain (Lithobates catesbeianus) for some time now. However, we don't have a standardized protocol for...
18 February 2021 3,453 4 View