Dear colleague

You can contact at RG Dr. María José Ranilla from University of Leon, Spain, that work with Rusitec. I already sent a RG message to her. Maybe she can help you. Best regards,

Redimio

Article The application of rumen simulation technique (RUSITEC) for ...

Thank tou very much dear colleagues

Thanks for your question on Rusitec. Before you start any practical application on using Rumen simulation technology, you will need to clearly understand how it works and the dynamics involved. These include; the nutrients required in the media, maintenance of anaerobic and the operation of continuous batch culture. Some scientists have tried using large scale batch fermentation simulating the rumen environment in the production of single cell protein (SCP) (similar to microbial protein synthesis) as a substitute of conventional protein sources such as beef and lamb but have encountered a lot of challenges due to the fact that SCP has a high proportion of nucleic acid material which has to be digested and metabolized through the Xanthine, Hypoxanthine, uric acid and allantoin pathway. With high levels of uric acid as intermediate metabolite you the risk of precipitating gout which crystallizes in the human body to sharp crystals that cause unbearable pain in the body. In the meantime, here are some good references from extensive work/review that was done by Czerkawski .

Czerkawski, J.W. 1976. Chemical composition of microbial matter in the rumen. Journal of the Science of Food Agriculture, 27:621-632.

Czerkawski, J.W. 1986a. An Introduction to Rumen Studies, Pergamon Press, Sydney.

Czerkawski, J.W. 1986b. Degradation of solid feeds in the rumen: Spatial distribution of microbial activity and its consequences. In: Control of Digestion and Metabolism in Ruminants. L.P. Milligan, W.L. Grovum and A. Dobson (eds), Prentice-Hall, New York, USA. pp 158-172.