Laura España Serrano

4 Questions 8 Answers 0 Followers

Questions related from Laura España Serrano

Which HUVEC cells should I use for tube formation assays?

I'm planning to use HUVEC cells for a tube formation assay in matrigel to measure the anti-angiogenic effects of some drugs. I have no experience with this assay and I found that there are...

19 February 2015 1,226 4 View

Does anyone have experience in non-radioactive EMSA? Is the efficiency similar to the p32 EMSA protocol?

I want to analyze possible protein-DNA interactions and I'll use EMSA, but I prefer not to use radioactivity if it's possible. I saw there are kits that use labeled probes with biotin in the 3'...

14 March 2014 3,246 9 View

What is the minimum number of reads needed to start the data analysis in a Chip-Seq assay?

I obtain around 10 million reads for each sample in my Chip-Seq assay . But after the Bioinformatics clean the sample, map and remove the duplicates we only have around 10% of the reads that we...

18 January 2013 2,793 3 View

Bioinformatic analysis of Chip-seq assay: do I need an Input sample to analyze the seq data? If so, how do I use it?

I did a triplicate of Chip assays, and I sent to sequence these 3 samples and one Input because I read I'll need it for the future bioinformatic analysis of the data. I don't have two different...

08 January 2013 6,010 9 View