Pasquale Termolino

10 Questions 9 Answers 0 Followers

Questions related from Pasquale Termolino

Does anyone know a DNA extraction protocol from starfish?

I tried different protocols but at the beginning of the procedure the tissue (starfish gonads) gelifies and blocks the other extraction phases.

25 May 2021 3,537 1 View

Setting up an HRM experiment to identify polimorphism in A.thaliana

Any good protocol and procedure method to make a successful HRM experiment in A.thaliana? I have to verify SNPs i already know in two population of plants to check genotype after KASP procedure.

30 June 2017 8,542 0 View

How do I control genetic maps quality?

I have a map of a plant BC1 population i have drawn with MAPDISTO software and re-drawn with joinmap4.1 software. Both maps show linkage between two arabidopsis chromosomes probably due to...

02 October 2015 4,569 5 View

I am trying to perform SAQPCR for copy number quantification of transgenes, can anyone suggest how I should proceed?

I developed a suitable plasmid for tomato, and I am trying to understand mathematics behind the technique. I need an example file to proceed.

14 April 2015 2,648 2 View

Is there a way to substitute QIAshredder column in plant RNA extraction kit?

I have a RNAeasy plant minikit and lilac columns are depleted, but I have plenty of pink columns. I would like to know if there is a way to avoid using QIAshredder columns. Thank you

25 February 2015 5,205 3 View

I have a problem with transformed tomato explants getting vitrified after regeneration - any suggestions?

I use a protocol with phytagel, maybe this is the problem?

19 June 2014 4,845 3 View

I have a soil sample and I want to distinguish how much of bacteria, fungi and plants are present. Any suggestions?

I need a precise detection technique to detect bacteria, fungi, plants in soil.

23 September 2013 7,105 2 View

Is it possible to recycle Whatman UNIFILTER microplates?

I have Whatman UNIFILTER microplates used for DNA extraction from Arabidopsis thaliana and I would like to know if there is a way to clean and re-use them (they are very expensive).

08 May 2013 9,345 11 View

Is it possible to use frozen tissue for Chip-seq?

I have tomato samples to use in a Chip-seq procedure. I can't process them immediately after harvesting and I would like to know if someone has experience with this kind of problem?

01 March 2013 5,626 4 View

I need a protocol to extract DNA massively from Arabidopsis thaliana.

Anyone that knows a very good protocol to extract milligrams of DNA from Arabidopsis thaliana? The starting tissue is not a problem, for the moment.

26 April 2012 8,269 2 View