We have single stranded aptamer DNA, we want to elute that aptamer by gel extraction kit (Sigma). This SS DNA (aptamer) could be eluted on the basis of pH? We have binding buffer (20mM Sodium phosphate, 500mM Sodium chloride, 5mM Imidazole, pH- 7.4) for SELEX round.

Can we use this binding buffer of SELEX as elution buffer, instead of Tris-buffer (which is mentioned is kit) during gel extraction procedure for elution of our SS DNA aptamer?

Thank you in advance.