Dear all,
I am facing problem to prepare 500 ml of 0.5 M EDTA for DNA extraction. I have dissolve 93.05 g Na2EDTA.2H2O in 350 ml MQ H2O, and then adjusted pH to 8.0 with 10M NaOH.
I am finding clear solution (350-380 ml) with very few undissovled salt (Na2EDTA) after adjusting the pH into 8.00. However, there is no further salt dissolution when I am adjusting the volume upto 500 ml, and the solution becomes opaque. It would be a great help if any of you could suggest me any valid reason of this and solution of the issue.
Thanks in advance for your kind support.
Kind regards,
Tanushree
Dear Mam, you may find some probable solutions here
https://www.researchgate.net/post/I-have-been-preparing-05M-EDTA-ph-8-since-yesterday-and-it-is-not-dissolving-I-need-help-coz-I-seriously-need-to-prepare-TAE-buffer
Many thanks dear, Abu Bakar. I have checked it. However, the problem is not solved yet.
I appreciate that you are seeing a problem, but you really shouldn't be! Disodium EDTA is normally very easy to dissolve and you should have a completely clear solution at pH 8. Are you keeping the pH probe in the solution while you adjust the volume? Are you sure that your pH meter is functioning correctly? The only explanation I can think of is that your pH is far lower than you think it is.
Many thanks Nick for your kind suggestion. I have found clear solution with a small amount of undissolved salt when the pH reached at 8.00. Though I did not keep the pH meter on during volume up, I have checked the pH after that. There was no change in pH. Therefore, I am confused about the reason. Would you please suggest me further?
Thanks in advance for your kind suggestion.
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