I am curious to know whether it is feasible to silence two unrelated/ related genes (eg: a transcription factor and a gene that it regulates downstream)consecutively in the same cell line( I mean in the same dish in which the cells undergone 1st silencing) using siRNA transfection method in commercially available cell lines?
Definitely possible. There is no mechanism by which after a cell accepts and takes in an siRNA, it is immune non-receptive to other siRNA molecules. Best of luck!
Hello Craig,
Thank you for your response, have you tried doing it before? I was trying to find some published articles on this I couldn't find any if you have any could you please share it with me to get an idea on it.
Hi preethi,
well, you can silence 2 genes simultaneously in the dish. However, I would strictly reccomend you to use suitable controls (other than vehicle control) which will help you to interprete your results in a better way.
read this paper (from my lab)! good example to silence two genes at a time.!!!
http://cancerres.aacrjournals.org/content/canres/70/24/10362.full.pdf
-- Best regards
Hai Kishore Ak ,
Thank you very much for your response will go through the paper.! all the best!
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology
- Culture Cells
- Cell Line