Preethi Sridharan

5 Questions 12 Answers 0 Followers

Questions related from Preethi Sridharan

Is it possible to silence two genes simultaneously using siRNA transfection method in cell lines?

I am curious to know whether it is feasible to silence two unrelated/ related genes (eg: a transcription factor and a gene that it regulates downstream)consecutively in the same cell line( I mean...

09 September 2018 7,235 4 View

Has anyone found an increase in gene expression using siRNA?

I am trying to knockdown gene of my interest in U251 and U87MG cell lines I found that after siRNA transfection my gene of interest showing upregulation upto 2 folds even after several...

09 September 2017 2,609 6 View

Does the picture (attached) shows that my nanoparticles are completely dispersed?

The nanoparticles of my interest were dispersed in milli q water (in milky white colour) but when I filter sterilized them using 0.22 uM filter the solution became clear (clear solution seen in...

07 July 2016 3,042 16 View

Does anyone have T98G (glioblastoma) cell line?

I am in need of T98G cell line for my work and NCCS doesn't have it. Could someone kindly send me a flask/ a cryovial if you possess

01 January 2016 284 0 View

Which is best reference gene for Real-Time PCR gene expression studies under chemical hypoxic condition?

I am trying to study the expression of my gene of interest under hypoxia (chemically induced) there were many reports that states that GAPDH and Microglobulin Beta are more stable genes that can...

08 August 2015 7,954 5 View