I am in need of T98G cell line for my work and NCCS doesn't have it. Could someone kindly send me a flask/ a cryovial if you possess
I am curious to know whether it is feasible to silence two unrelated/ related genes (eg: a transcription factor and a gene that it regulates downstream)consecutively in the same cell line( I mean...
08 September 2018 7,184 4 View
I am trying to knockdown gene of my interest in U251 and U87MG cell lines I found that after siRNA transfection my gene of interest showing upregulation upto 2 folds even after several...
08 September 2017 2,553 6 View
06 July 2016 2,984 16 View
I am trying to study the expression of my gene of interest under hypoxia (chemically induced) there were many reports that states that GAPDH and Microglobulin Beta are more stable genes that can...
07 August 2015 7,899 5 View
I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...
03 March 2021 4,116 4 View
02 March 2021 3,060 3 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View
28 February 2021 9,936 2 View
I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...
25 February 2021 3,030 1 View