I add nanoparticle and Albumin together, incubator 24 hours. I run SDS gradient gel with this combination after 10min, 70 degree denature. I got something like half way waterfall on the gel.
Does anyone have suggestion for my problem? thanks,
I think there are several critical points:
- Do you know the size distribution of your nanoparticles. I think that they are not uniform in size. If the smaller ones are able to enter the gel you will not get clearly separated fractions.
- I assume that most of the particles are even too large to enter the gel.
- Is binding of albumin to your nanoparticles (which type) disturbed by SDS?
Peter
I think it'll likely depend on the nature of your nanoparticle (composition, ligands, size, etc.). I've run nanoparticle-protein conjugates on agarose gels before and they traveled quite nicely and tended to separate into bands based on the number of proteins bound. However, the nanoparticles I used have anionic surface ligands and are also conveniently fluorescent (making imaging in an agarose gel trivial). More information about your specific situation could help.
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