Hi
I performed organoids cultures in floating conditions. I need to know if the matrigel is necessary to maintaining and expanding the iPSC before differentiation. Could be replaced by cheaper support?
Thanks!!!
Dear Montse,
Yes you would always need a suitable matrix for maintaining your iPSCs prior to differentiating them into organoid conditions.
There are other matrix options available for feeder-free iPSC maintenance.
Geltrex, Laminin (esp laminin 521), CellStart, Vitronectin are few of the commonly used ones other than Matrigel.
If you decide to switch the matrix , it will be crucial to test different matrix and confirm which matrix is most suitable for your iPSC line.
Therefore, I would recommend to keep a backup of your cells on Matrigel till you have nicely adapted the iPSCs to a new matrix of choice.
Good luck !
Hi Montse, to add to the first response - geltrex is a very good alternative to Matrigel (made from the same source cells and covers the same mix of ECM proteins. Typpically Geltrex is more concentrated than Matrigel, so needs some dilution proir to adding to cells, to match the protein concentration you have used Matrigel at)
Alternatively - we recently launched a suspension culture system for Human PSCs (called 'StemScale'. cat # A4965001) which you can use to grow the PSCs without matrix and expand the cell numbers greatly prior to setting them up for differentiation into any cell type you desire. feel free to reach out to me if you would like to discuss trialling it. Hapy to help, Tony
There are other matrix are available in the market. In our lab for iPSC cell line culture, direct reprogramming of fibroblast to neuron culture, we use matrigel. It works perfect.
Hi Masuma,
I noticed your comment about matrigel alternatives, have you considered Type-0 marine collagen from Jellagen? A non-mammalian Matrigel alternative.
We have comparative data we would be happy to share. We have stock on the shelf and would love you to try it, please DM one of our product specialists, [email protected] if you want to know more.
Kind regards,
Andrew
PHA is a cheap option :
10 branched poly(Arg, epsilon-Lys)-hyaluronic acid hydrogel (PHA, for 3D cell culture)
Xenogenic-free, chemically defined, highly reproducible and excellent biocompatibility Without crosslinker and organic solution Mimic the natural extracellular matrix(ECM). Proper mechanical strength Storage at room temperature No need to pre-treat prior to using with cell suspension To perform assays on isolated spheroids, dissolve PHA with iso-osmolar trisodium citrate. Mesh size 50, 100, 150μm
83USD/10 pills
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