23 November 2019 3 3K Report

Hello,

I am performing immunofluorescence of HepG2 cells. I believe that some of my stocks may be contaminated with Mycoplasma because when I stain them, their membranes and portions of their cytoplasm are blue as well as the nuclei. Is it possible that I have simply over-incubated with DAPI to produce this non-nuclear signal? Also, why is DAPI unable to stain mitochondrial DNA? Is it unable to permeate mitochondrial membranes? These membranes should also be opened by Triton X-100, correct? Also, I should add that in the case mentioned above of membrane staining with DAPI, I reduced my Triton-X-100 (0.5%) incubation from 10 minutes to 1 minute because I am attempting to visualize a membrane-bound protein.

Thank you!

More David Schad's questions See All
Similar questions and discussions