Why not, free drying depend of vaccum or pressure not affect to antioxidant enzyme activity, freezing inhibit enzyme activity

Thank you Eshak

Hi, i completly disagree with ishak, yes freezing inhibit enzyme activity, but also denaturing enzymes and can modify the binding sites of those enzymes, so how could you measure the activity .....i would say activity....what is enzyme activity???? so we couldn't say freezing inhibit activity, decreasing temperature inhibit activity but freezing really i'm not sure.

Hi. The best method for preventing of enzyme denaturing is freezing in liquid nitrogen. by freezing we can protect enzyme.