Dear All
Why are S. aureus and E. coli selected as target strains for most of the research study on antimicrobial activity measurement? Is it this strain easily react with antimicrobial compounds?
Regards
Azlan
I think because they are highly and more frequently resistant.
S.aureus is a representative of gram-positive bacteria and E.coli - gram-negative ones. It is not related to the reaction of compound, more related to the pecularities of bacterial cell wall.
A very significant reason is precedent. They're typically cited in old, established protocols of regulatory significance that are accepted in the respective application. E. coli is not offered in EPA antimicrobial testing protocols and where offered in others, it's usually ATCC 8739 - about 70 years or more from original isolation - S. aureus is usually ATCC 6538 - approaching century from isolation. Above suggestions may have justified their original indication but you have to wonder if these are now lab creatures of questionable relevance to current environmental and clinical isolates.
https://www.epa.gov/pesticide-analytical-methods/antimicrobial-testing-methods-procedures-developed-epas-microbiology
It is also said that antimicrobial compounds that inhibit in vitro grown of S. aureus and S. pneumoniae, could also have antimicrobial activity over Mycobacteirum tuberculosis (even MDR). Something to start working on!
Dear Azlan:-
Staphylococcus aureus and Escherichia coli are always selected as a target for antimicrobial susceptibility because both of two bacteria are rapid growing microorganisms (They have short generation time, about 16-18 minute. The antimicrobial activity to be fully, it needs rapid growing microorganism (the optimum activity in mid logarithmic phase in bacteria like E. coli and S. aureus). Best Regard
The main reason is because both bacteria have short generation time. In addition, each represent gram positive and gram negative bacteria
There is no scientific base to choose both E.coli and Staph. aureus.There are a number of sensitive bacteria could be choosed for certain reasons.For instance,Listeria monocytogenes is choosed as it grows in different environments,different pH values, different temperatures,at high salt concentrations and its inhibition is promising.Bacillus cereus or subtilis are choosed as they are endospore forming.Clostridia because of its growth at anaerobic conditions and acute toxigenicity.Thus ,S.aureus as it is forme MRSA and MISA and VRSA strains. E.coli as it is entero invasive and enterohaemorhagic and one oportunistic pathogen of the family Enterobacteriaceae.SO,any organism has its specific discussion.
I think its Enterococcus spp. (compare to S aureus) and Escherichia coli are the most commonly and routinely selected as a representative of gram-positive and gram negative bacteria respectively for most integrative surveillance and monitoring program. Because both bacteria serves a s indicator bacteria for monitoring the selective pressure due to antimicrobial use. Check NARMS, CIPARS, CDC reports together with Campylobacter and Salmonella formed the core integrative surveillance bacteria for AMR monitoring
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