Hi. Anyone have come across this issue where the baseline is shifted like this when running the hplc? Is it due to contamination or else?

Mobile phase used is 5mM sulphuric acid , type of column is Bio-Rad Aminex

09 July 2020 9,321 7 View

Hi. Do you have any idea why the peak looks like that as I guess this is the solvent peak produced?

I have run the levulinic acid standard through hplc-refractive index detector with column and detector temp at 63 and 50 degree respectively. Mobile phase used is 0.005 M H2SO4 at flow rate 0.6...

12 November 2019 6,348 2 View

How Transfer data/image from GPU to FPGA using MATLAB ?

Currently I Already did my work to transfer image on GPU and CPU. Apart from that, i try to expand the work with FPGA. Unfortunately, i still searching the suitable method how communicate CPU and...

10 October 2018 2,997 3 View

What is the significance of soil microbial biomass N:P value in the agricultural soil?

The microbial biomass ratio is considered as an important indicator of microbial nutritional balance. High microbial biomass C:N ratio for example, usually indicates unbalanced nutritional...

12 February 2018 1,012 6 View

Which oneis better QTOF or QTRAP?

Dear All Which one is better to determine phenolic compound inside the dragon fruit peel either use QTOF or QTRAP? ESI QTRAP is normal resolution but with ion trapping for better spectrum...

21 April 2017 1,180 7 View

Is there any relationship between antioxidant and antimicrobial activity?

Dear All Kindly to ask you, is there any relationship between antioxidant and antimicrobial activity? since I already extract active compound from dragon fruit peel using distilled water as a...

01 September 2016 4,511 14 View

How to write a good thesis abstract?

What are the components should have in writing a clear and good abstract?

06 June 2016 6,780 24 View

Hi, one question related to correlation. Is it possible for me to execute correlation with a sample n=24 to see the relationship between variables?

26 April 2016 3,619 28 View

What is the recommended amount of PCR product to load on DGGE?

I performed PCR which targets bacterial 16S (using GC968f/1401r) and fungal 18S (using NS1/GCFung), each produced ~400 bp and ~350 bp, receptively. The DNA was extracted from soil sample.Can...

22 April 2016 3,134 3 View

No title

My soil sample is of sandy loam. I've done the extraction protocol several times, precisely as stated in the manufacturer's instruction manual but I did not get any good result. The concentration...

18 March 2016 1,779 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

How can I verify my centrifugal fan design for electric motor cooling?

I have radial blade centrifugal fan design which will be mounted on exhaust side of electric traction motor. The fan will be mounted on same motor shaft inside the housing. How can I verify that...

23 February 2021 2,390 3 View

How can I improve the concentration of banana fruits extracted RNA?

Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...

22 February 2021 6,126 2 View

Are ZnS nanoparticles moisture sensitive?

Hello Everyone, I tried to form ZnS nanoparticles using the hot injection method and was able to form the same (confirmed by XRD). When I centrifuged, after the reaction I got a kind of white...

17 February 2021 1,709 3 View

Does calculating the interaction index between a drug and a food matrix make sense?

Dear all, I'm conducting the revisions of some of my manuscript, with is the impact of the combination of Aspirin and a fruit juice in colorectal cancer cells. Two of the reviewers have asked to...

16 February 2021 3,141 3 View

Peptide precipitation as pre-step for acid hydrolysis for amino acid analysis?

I'm currently experiencing problems during acid hydrolysis for total amino acid analysis as my first samples contain high amounts of sugar (I get too low results back). I therefore had the idea to...

14 February 2021 6,049 3 View

What is the optimal propagation regime for pET-1M(KanR) and pMCSG7(AmpR) plasmids in DH5alpha E.coli?

Dear all, I am trying to harvest the low-expression plasmids in the mentioned E.coli strain. I constantly gel very low concentrations (30-50 ng/uL while the concentration is usually around 100...

08 February 2021 4,255 4 View

Why is there cloudy white solution in flavonoid test tube?

I do flavonoid test for biscuit incorporated with muntingia fruit powder. Sample extracted with metanol (1:10 ,w/v), macerated 24h, sentrifuged and filtered. Supernatan used for analysis. I have...

08 February 2021 6,779 4 View

Why do we wash azolectin with acetone?

I want to prepare liposomes made of azolectin (sometimes spelled as asolectin). I start with the azolectin powder, and many protocols say that I should wash it with acetone (dissolve, centrifuge,...

04 February 2021 2,738 3 View