Is it possible to make a buffer with range above pH 11?

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Can anyone suggest me the best way for Regression analysis of Accidents?

Dear Altruist, I want to make a regression analysis based on accident data. But I am not finding the necessary information about what type of regression is more applicable for analysis accident...

23 February 2021 9,956 3 View

Which alginate is best for making gel for wound dressing application?

We are looking into utilizing alginate as a gel coating on an HDPE substrate for drug delivery. I would like to ask the community about their experience with alginate and which commercial sources...

15 February 2021 4,319 4 View

How to know initial values for non-linear regression?

Does anyone know a reliable methodology to estimate the initial values of theta1 (or A) and theta2 (or B) in a non-linear model? For example, a = (A+Bx/hc)(hc/x-Eg) How to reliably estimate the...

17 January 2021 1,327 1 View

How can i get AMPS-1D resources?

I am trying to model a photovoltaic device using AMPS-1D software. But once I am submitting it to queue, it remains there for infinite amount of time without giving the result. I couldnt...

11 January 2021 1,829 1 View

Solvent for extract for MTT assay?

I have a problem with solvent for extract for MTT assay. This extract made from n-hexane solvent. I have used DMSO for solvent before it mixed with culture medium, but the extract didnt dissolve....

23 November 2020 8,514 7 View

How to increase the efficiency of overexpression using lentiviral TET-ON system in primary kidney cancer cells?

I am using a lentiviral TET-ON system for conditional overexpression of my gene of interest in primary kidney cancer cells. I use viral load from transfection of HEK293T cells after 48 and 72...

16 November 2020 2,496 1 View

Is artificial intelligence (AI) dangerous for the existance of human civilization?

At present, AI technology is reducing the human tasks and replacing the persons from their workplace. In future, it may completely replace the human in all the sectors.

26 April 2020 7,087 27 View

How to calculate total electronic cross sections?

Hello, I'm trying to understand photon shielding parameters such as mass attenuation coefficients (MAC's) and effective atomic numbers (Zeff's). I would like to ask how to calculate for total...

08 April 2020 7,893 2 View

How to check qRT-PCR data distribution?

hi, i would kindly ask you for help, because i have been strugling with this issue for few weeks. Basically i would like to know how can i check if my qRT-PCR data has normal distribution so i can...

20 January 2020 3,371 2 View

Why are there no mitotic cells seen in our onion root tip assay?

We're researching the cytotoxic potential of a plant extract using the onion root tip assay. We have 4 set-ups; 75%, 50%, 25%, and the negative control (water). We let the onions grow for 48 hours...

07 February 2019 159 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View