Hi,

I'm currently working on the analysis of wastewater treatment efficiency on viruses. I have spiked my wastewater (abbatoir water from fish slaughterhouses) with BCoV and TV prior to treatment with standard chemical coagulants containing iron and aluminum, and will now evaluate the efficiency by RT-PCR.

The issue is that my RT-PCR results are fluctuating and are often resulting in No Cq, especially on the treatments where max amounts of iron are used. I think the problem might be innhibitors from the coagulants. I'm currently using QiAmp viral-RNA isolation kit and RNeasy clean-up kit.

Has anyone working with samples containing chemical treated wastewated experienced the same issues? If yes, do you have any recommendations to other kits/procedures I can use to clean my samles sufficiently to isolate the RNA?

Thank you in advance.

Best,

Mia Tiller Mjøs