Why crystal like structures are formed on NGM plates cultured with E. coli OP50?

When I prepare NGM plate for the growth of C. elegans, the plates are clear. However, when I spread culture of E. coli OP50 on NGM plates and incubate at 37 C overnight, I observed crystal like...

10 November 2017 707 4 View

Any advice on the difference in the Ct value of reference gene in treated and control samples?

I am using 16S primers for qPCR. However I got different Ct values in may treated and control sample (30.08 and 28.85). Would it lead to considerable error in final results? In my opinion,...

06 July 2015 3,326 10 View

Is it necessary to dilute cDNA before qPCR? How much dilution should be made? How to determine best dilution factor?

Kindly explane importance of cDNA dilution before qPCR and how one can determine best dilution factor for cDNA samples? How it will effect results of qPCR? In other words, how much quantity of...

05 June 2015 1,350 22 View

Is it possibile to determine genomic DNA contamination in cDNA sample on the basis of qPCR results?

Is there any possible way to find out genomic DNA contamination/residues in cDNA by analyzing results of qPCR? Can we get any information related to genomic DNA by Ct value?

05 June 2015 738 4 View

Which are experimentally proved Best house keeping genes to study gene expression in Pseudomonas syringae?

I need some information about house keeping genes to be used as internal control particularly for Pseudomonas syringae. Kindly let me know which house keeping genes are good to study gene...

31 December 2014 5,607 4 View

How can I avoid contamination of Genomic DNA during RNA extraction?

It is observed that RNA samples are often contaminated with genomic DNA. How can this contamination be avoided? What are the possible causes for genomic DNA contamination during mRNA extraction.

31 December 2014 5,040 3 View

We want to remove the gaps/errors of our laboratory genomes with some reference genomes. Which software would be the best option?

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09 October 2014 9,468 15 View

How can one convert genome sequence files of microsoft office into genbank format?

I have a genome sequence however, sequence is in microsoft word file and annotation is present in excel file. How can I convert/merge this information into genbank format. (sequence is not...

09 October 2014 6,644 6 View

Which are the best expression vectors for Pseudomonas syringae?

I need some good expression vectors for Pseudomonas syringae for over expression of proteins and for the construction of complementary strain. along with that, I also need the source/link from...

08 September 2014 5,360 4 View

What software/database could be used for identification of pan genome/core genome of user's own sequence data?

1) The sequence data is in contigs form. 2) Please suggest windows based tools. 3) Important literature/source would also be appreciated.

08 September 2014 8,349 18 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View