Dear all,
I want to express my protein of intterest in absence of salt. Now the challenge is to see the survival of cells and even if it survives, where I could find a cell medium (any company that provide it). Please recommend if any commerically available media that has no salt.
Thank you
With kind regards
Prem
check this out:
https://www.researchgate.net/post/Salt-free_bacterial_growth_media
Maryam Ghobakhloo Thank you for your response. But I need the salt free media for mammalian cells. If you could suggest any that would be great.
Thanks again !
Prem
Hi Prem
There are several salt-free media for mammalian cell culture, including DMEM w/o NaCl, IMDM w/o Na/Cl, etc. However, salt-free media refer to the one without NaCl, they may still contain other salts such as KCl. These salts are often necessary for cell growth and function. Thus, I'm afraid there may be no completely salt-free media.
Maryam Ghobakhloo Is it possible to make the media using all the composition seperately and do not add the salt in it ? If yes where would get those composition for mammalian cells (suspension/adherent).
Thank you
With kind regards
Prem
I don't think mammalian cells can survive in a medium that doesn't contain any salt. The presence and concentration of salt are required for cell signalling like sodium-potassium pump, calcium pump etc, and also for proper osmotic pressure because the media can be hypotonic leading to swelling of cells.
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