Is it possible to do a No Reverse Transcriptase control with the Luna One Step qRT-PCR Kit?

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Does this corrosion exam question read well and do you see the solution pathyway?

Hello, I am teaching for my first time this semester. In writing my exam, I felt pretty good about most of my questions except one. I was wondering if anyone could provide feedback on their...

28 February 2024 9,051 1 View

What should I do with missing data when averaging variables?

I am performing a cross-country regression analysis with a sample of 101 countries. Most of my variables are averages of annual data across a period of 7 years. Every one of my primary variables...

20 April 2023 4,337 3 View

What terms are the International Monetary Fund's International Financial Statistics in?

I am using certain IFS variables in my research, particularly "Supplementary Items, Direct Investment, Net Incurrence of Liabilities (Excluding Exceptional Financing), US Dollars" and...

30 March 2023 5,534 4 View

How do we decide on the sample size in a particular study?

I plan to study teachers' perception on M-Learning using a survey. How many teachers should be involved in the survey?

06 February 2023 8,285 8 View

What causes this IHC artifact?

Has anyone seen this immunohistochemistry artifact, and do you know what causes it? The artifact appears as patches of chromogen throughout the tissue sections. I have seen this artifact in...

11 December 2022 744 2 View

I'm having a lot of trouble dissolving a phthalate/vehicle combination in cell culture medium for in vitro toxicity assays; Can anyone provide tips?

I work with a very hydrophobic phthalate (DEHP). Visually, it appears to be miscible in DMSO and 100% ethanol but following addition of the solvent/solute combo to culture medium (RPMI-1640 +...

08 September 2022 9,900 0 View

ANSYS Fluent - How to get zero species diffusive flux through reaction wall, or specify species flux through a wall?

I am using ANSYS Fluent to model a 2D microfluidic channel. The channel is 50um tall and 1.5cm long. This model is of cells on the bottom surface consuming oxygen and producing dissolved waste...

08 August 2022 627 0 View

What to do with 100% datapoints when conducting a Logit transformation?

I have a panel dataset of 120 different countries measuring a variable over three periods. This variable indicates the percentage of 1000 respondents in each country that answered yes to the...

01 February 2022 1,284 7 View

How do I prepare zebrafish embryos for homogenization?

Hello, I am wanting to do an RNA isolation on zebrafish embryos using the PicoPure RNA isolation kit. I was wondering if anyone could describe the homogenizing procedure for zebrafish embryos...

16 January 2022 6,773 0 View

How does subsequent heating affect properties of previously cured epoxy?

If epoxy is cured at low temperature to 50% degree cure, then further cured to 100% at higher temperature, how will that be different than curing to 100% at the higher temperature? Similarly, how...

20 July 2021 8,007 5 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View