How much RNA should i prepare from my samples for sequencing?

Hi everyone I'm going to extract RNA for sequencing. I want to send the samples to the BGI company for sequencing. How much RNA should I prepare from my samples?

03 April 2018 4,604 3 View

Is there someone who guides me to extract a high quality RNA?

Hi everyone. I have to extract RNA from a tissue. Is there someone who guides me to extract a high quality RNA ? ( Important points that must be met, Suitable kit and protocol)

10 November 2017 8,859 8 View

Can someone introduce me an paper or website for whole genome sequencing?

Hi everyone I need an article or website for how whole genome sequencing of a species (Workflow, registration, annotation, materials and methods and etc). Can someone introduce me an article or...

09 October 2017 3,040 6 View

What method can be used to obtain the genome of a bacterium from water and milk that has been contaminated with the bacteria?

I'm going to use PCR method for amplification of a gene from a bacterium. my examples are water and milk That have been infected by a bacterium.Is there a special kit for the isolation of...

04 May 2017 9,363 8 View

How Be used from Circular Dichorism in the drug recombinant proteins?

Hello everyoneI need complete information about CD description in the drug recombinant proteinsIs there anyone who can help me?

31 December 2016 678 8 View

Is the Isoelectric Point of the capsid the same of the protein or does it change?

hi I am expressing a Virus Like Particle in Baculovirus System. The virus express a protein that self-assembles itself in a capsid. I know th IP of the protein. Since only one of three domains of...

31 December 2016 5,080 1 View

In your opinion, What is the best Human housekeeper genes for use in Liver tissue?

hi I am testing the housekeeper genes in the liver in humans, so I use a technique such as SYBR Green. In your opinion, what is the best genes?

31 December 2016 394 7 View

Is there general signal peptide for plants?

hi signal peptides are commonly protein-specific, I wonder if there is "general" signal peptides which can be used among plants. Hereby, it would enable more convenient protein expressions in...

31 December 2016 1,711 5 View

What is method of finding the cpg islands in promoter region of a gene?

31 December 2016 136 4 View

Can anyone recommend a method to determine if bacteria are hup+ or hup-?

hiI want to measure N fixation as a H2 evolution with a specific device and I need to know if my strain has an active hydrogenase enzyme.

31 December 2016 4,146 2 View

Why should we choose deep learning to machine learning? Why is CNN better for image classification? Can I get Typical Answer?

What Characteristics makes CNN work better?

03 March 2021 1,458 4 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

Is there a classification scheme for rock cavities or caverns with respect to their size and shape?

The range of forms and sizes of cavities in rock is very big. Is there a classification scheme for rock cavities regarding their size and shape?

02 March 2021 4,511 3 View

Image Processing and classification using keras/Tensorflow?

Do you have some recommendations approaches on Image processing and classification of plant diseases using TensorFlow/Keras?

02 March 2021 1,493 4 View

Does anyone have kinetic and kinematic dataset for gait classification?

I want to differentiate between normal and abnormal movement. For this reason, I am looking forward to this type of dataset.

01 March 2021 9,485 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Where can find Medical Image Data Sets for Machine learning research project?

I want to work on medical image(disease) detection and classification. Where can I found Image data sets?

24 February 2021 3,130 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View