I would like to calculate diversity index from DGGE banding patterns.
Can the DGGE band strength/intensity actually be estimated visually as described by Gafan et al. (2005)?
Anyone knows any papers that make use of the same approach?
Reference:
Gafan, G. P., Lucas, V. S., Roberts, G. J., Petrie, A., Wilson, M., & Spratt, D. A. (2005). Statistical analyses of complex denaturing gradient gel electrophoresis profiles. Journal of clinical microbiology, 43(8), 3971-3978.
Article Statistical Analyses of Complex Denaturing Gradient Gel Elec...
Hi Muhammad,
I think the authors chose visually estimation maybe because they had no better methods at that time. The software, such as Quantity One, should be not such popular like today. In my private opinion, I think the software-based analysis would be much more sensitive and accurate than our eyes. So I recommend that you should better use software (e.g. Quantity One) to analyze your DGGE bands.
regards,
Guangshan
- Badges
- Science method