Muhammad Farid Azlan Halmi

8 Questions 4 Answers 0 Followers

Questions related from Muhammad Farid Azlan Halmi

How to remove residual broth media from lignocellulose (biochar) powder for FTIR analysis?

Briefly, cellulolytic bacterial cultures were inoculated into a flask containing minimal media (containing K2HPO4, KH2PO4, MgSO4.7H2O, NaCl, NaNO3 and CaCO3) supplemented with biochar (as sole C...

27 November 2018 8,918 3 View

What is the significance of soil microbial biomass N:P value in the agricultural soil?

The microbial biomass ratio is considered as an important indicator of microbial nutritional balance. High microbial biomass C:N ratio for example, usually indicates unbalanced nutritional...

13 February 2018 1,218 6 View

How can enzyme activity results from sphectrophotometric assay be corrected to take into account the absorbance by biochar?

I realize that some authors used different standard curves in spectrometeric/ colorimetric enzyme assay in order to account for the adsorption that some of the biochar had on the product of the...

06 August 2017 3,197 1 View

Is it possible to analyse DGGE banding patterns manually without the aid of software?

I would like to calculate diversity index from DGGE banding patterns. Can the DGGE band strength/intensity actually be estimated visually as described by Gafan et al. (2005)? Anyone knows any...

30 October 2016 1,995 1 View

Which indices is the best for clustering analysis of DGGE band patterns?

I want to perform clustering analysis of DGGE band patterns (presence/absence data) using UPGMA algorithm. The distance matrix can be computed using different indices/coefficient (Pearson, Dice,...

17 May 2016 360 2 View

What is the recommended amount of PCR product to load on DGGE?

I performed PCR which targets bacterial 16S (using GC968f/1401r) and fungal 18S (using NS1/GCFung), each produced ~400 bp and ~350 bp, receptively. The DNA was extracted from soil sample.Can...

23 April 2016 3,319 3 View

I used PowerSoil® DNA Extraction Kit (MOBIO) to extract DNA directly from soil but it was unsuccessful. Which step of the protocol can be modified?

My soil sample is of sandy loam. I've done the extraction protocol several times, precisely as stated in the manufacturer's instruction manual but I did not get any good result. The concentration...

19 March 2016 1,996 2 View

What is the significance of microbial biomass for carbon and nitrogen mineralization?

Microbial biomass changes rapidly in response to changes in soil properties, but how to interpret the increase or decrease of microbial biomass carbon and microbial biomass nitrogen in relation to...

29 January 2016 5,756 1 View