Guangshan Wei

3 Questions 10 Answers 0 Followers

Questions related from Guangshan Wei

Can I use relative abundance data of OTUs to calculate the alpha- and beta-diversity of functional gene-based data?

After high-throughput sequenceing of 16S rDNA, the sequencing depths of different samples usually vary a lot. The sequencing depth can affect alpha and beta diversity analysis, therefore, we...

01 December 2016 9,826 4 View

How to do a scientific sampling when we perform a microbial diversity investigation on natural environments, such as soil, seawater etc.?

There are so many microbial diversity investigations using high-throughput sequencing on variety of natural environments, e.g. land soil, fresh and salt water, sediment etc. Some of them sampling...

21 November 2016 5,038 4 View

When we analyze bacterial 16S rDNA sequences after NGS, if the OTUs which have only one sequence should be removed?

After high-throughput sequencing, we obtained thousands of 16S rDNA reads for each sample. When we analyzed these sequences, firstly, we performed quality filter to remove the low quality...

03 November 2016 3,470 7 View