I want to ask some basic questions about adoptive transfer:
1. Are T cells from Thy1.2 mice immunogenic in Thy1.1 mice?
2. I want to transfer CD8 T cell from Thy1.2 mice (my knockout mice) to Thy1.1 WT mice without irradiation, and then compare the T cell response to virus infection of knockout T cell and WT T cell, is that possible? My question is whether the transferred T cell can survive and respond to virus challenge? Any suggestions will be welcome.
3. The C57BL/6 mice we routinely use is Thy1. 2, is that right?
Dear Junjie, I noticed your question only now as I have the same problem..... did you solved it?.... anyway, the answer to the third question should be YES: the normal C57BL/6 should be Thy1.2 (I asked to Harlan expert about).
Hi, Francesco,
I still didn't have the answer.
I didn't get started to do the adoptive transfer. I will keep you updated if I get the answer.
Dear Junjie, please, give a look to this article.... it seems to me that they transfer Thy1.2 into Thy1.1 (Fig.4)
http://www.plosone.org/article/info:doi/10.1371/journal.pone.0004089#s3
May be this paper will help you.. I am also trying to understand the same thing. If I could transfer thy1.2 T cells to Thy1.1 recipient without irradiation...
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3024899/
Ive recently read a publication in which they made an in vivo adoptative transfer of Thy1.2+ OTII or OTI cells unto a Thy1.1+ recipient mice without describing that they did the radiation. Here the link to the article, perhaps you find it useful?.
http://jem.rupress.org/content/210/9/1855.full
And all the mice, as they describe in the materials and methods part, were bred under the C57BL/6 background
I believe you may be able do this transfer in mice which are H2 compatible. In this case H2b. I have not worked with Thy1.1WT.
If the transferred cells will receive stimulation from the viral antigens they should survive (and proliferate!) without the need of previous irradiation.
Thy-1.2 is potentially immunogenic in Thy-1.1 mice (and vice-versa) but I have never observed it in my experiments.
Of course, this is only true if both mice are from the same gender, same background (C57BL/6) and the genetic alteration on the transferred cells does not elicit an immune response against it (say, if it expressed ovalbumin!).
Hi, just want to update my test.
I have tried to transfer Thy1.2 CD8 T cells into Thy1.1 mice and then I challenged the mice with virus. After two weeks I collected the spleen T cells and I did recover a very small population of Thy1.2 cells (1-2%). Importantly they showed antiviral CD T cell response by tetramer staining and can reduce viral infection to some extent.
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