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Questions related from Pedro Veliça
I've been culturing T cells for the good part of 8 years now and have always used standard RPMI supplemented with serum and 2ME. I was told recently (in a review process) that it " is generally...
26 February 2019 7,279 1 View
I would like to model CAR-T cell therapy using an entirely mouse system, that is, a syngeneic model whereby mouse (ideally C57/Bl6) T-cells can be transduced with an anti mouse CD19 CAR and...
12 December 2017 4,444 1 View
The FACS machine at our core facility has a 670 LP (long pass) filter to detect PerCP and PerCP-Cy5.5. Yet, unlike the band pass (BP) filters these seem to accept a large chunk of the emission...
17 February 2015 1,782 5 View
Does the gender of the recipient animal matter for the engraftment of the B16 tumour line? What is the gender of the cell line? I have seen publications were males were used and others were female...
09 February 2015 6,035 17 View
What type of assays can one do to measure lung function in mice?Can you measure oxygenation of blood? Breathing volume? Inflation of the lungs?
15 January 2015 1,366 9 View
I have read that GFP can be expressed from a HIF-dependent promoter so that the cells become GFP+ in hypoxia but can is it possible to encode a hydroxylation motif on the GFP protein itself in...
31 December 2014 3,082 3 View
Has anyone measured surface expression of PDL-1 on EL4 cells? And while I am it: do they produce TGF-beta? Just wondering what kind of immunosuppressive "arsenal" EL4 cells have to fight off...
22 January 2014 2,194 9 View
The idea would be to use retroviral vectors bearing LoxP sites to infect cells expressing inducible Cre recombinase (under control of Dox or Tamoxifen). This would be used to delete transgene...
19 June 2013 1,786 8 View
