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Questions related from Pedro Veliça
I've been culturing T cells for the good part of 8 years now and have always used standard RPMI supplemented with serum and 2ME. I was told recently (in a review process) that it " is generally...
26 February 2019 7,312 1 View
I would like to model CAR-T cell therapy using an entirely mouse system, that is, a syngeneic model whereby mouse (ideally C57/Bl6) T-cells can be transduced with an anti mouse CD19 CAR and...
12 December 2017 4,479 1 View
I have routinely conditioned C57BL6 mice with 5.5 Gy of total body irradiation (TBI) to achieve non-lethal temporary myeloablation.What would be an equivalent chemotherapy regimen?
17 March 2016 4,138 1 View
I've been analysing the immune composition of spontaneous breast cancers from the MMTV-PyMT model using flow cytometry.I routinely find that a proportion of CD8 T cells in the tumour (but not in...
05 January 2016 7,089 6 View
I've been activating purified mouse CD8 T cells with CD3/CD28 beads to measure CFSE dilution and CD44/CD62L expression.When it comes to flow cytometry I use a live/dead stain to gate my live...
27 May 2015 2,934 7 View
In this model, carcinogenesis is induced in breast tissue by inducing the Polyoma Middle T antigen with the MMTV promoter resulting in tumors that develop over the course of 15-20 weeks that mimic...
24 April 2015 1,022 4 View
The FACS machine at our core facility has a 670 LP (long pass) filter to detect PerCP and PerCP-Cy5.5. Yet, unlike the band pass (BP) filters these seem to accept a large chunk of the emission...
17 February 2015 1,825 5 View
Does the gender of the recipient animal matter for the engraftment of the B16 tumour line? What is the gender of the cell line? I have seen publications were males were used and others were female...
09 February 2015 6,072 17 View
What type of assays can one do to measure lung function in mice?Can you measure oxygenation of blood? Breathing volume? Inflation of the lungs?
15 January 2015 1,403 9 View
I have read that GFP can be expressed from a HIF-dependent promoter so that the cells become GFP+ in hypoxia but can is it possible to encode a hydroxylation motif on the GFP protein itself in...
31 December 2014 3,116 3 View
Has anyone measured surface expression of PDL-1 on EL4 cells? And while I am it: do they produce TGF-beta? Just wondering what kind of immunosuppressive "arsenal" EL4 cells have to fight off...
22 January 2014 2,228 9 View
For the last couple of years I have been using the EL4 thymoma cell line for in vivo tumour challenges. We use the modified EL4 line that expresses the flu NP antigen, recognised by our model F5...
23 December 2013 3,041 5 View
The idea would be to use retroviral vectors bearing LoxP sites to infect cells expressing inducible Cre recombinase (under control of Dox or Tamoxifen). This would be used to delete transgene...
19 June 2013 1,823 8 View
