4 Questions 7 Answers 0 Followers
Questions related from Junjie Zhang
Hello Dear colleagues, I need some help with NFATc2 (NFAT1) westernblot in Jurkat T cells. I induced Jurkat T cell activation with CD3+CD28 antibody and goat-anti-moue IgG then collected the cells...
26 January 2016 3,430 3 View
I am trying to collect Bronchoalveolar lavage from mice. I learned it from another lab where they used needles to insert into the trachea. But when I was trying, the liquid could leak and the...
27 February 2013 1,422 7 View
I want to ask some basic questions about adoptive transfer: 1. Are T cells from Thy1.2 mice immunogenic in Thy1.1 mice? 2. I want to transfer CD8 T cell from Thy1.2 mice (my knockout mice) to...
18 February 2013 1,162 9 View
I am doing peptide stimulation of spleen T cell and IFNr staining. Here's my protocol, I thaw the spleen cells (I have already done tetramer staining so I am sure around 10% of the CD8 T cell...
23 January 2013 6,813 3 View