Francesco Nicoli

3 Questions 13 Answers 0 Followers

Questions related from Francesco Nicoli

Does anyone have experience with KLRG1 staining in humans?

In my experience it worked quite well in mice. Now that I want to see it in humans, it seems that all literature uses an antibody developed by a professor from Freiburg (Germany), and the...

12 May 2015 9,152 2 View

Does anyone have experience on RNA extraction from flow through liquid of DNA separation kit?

We are interested in separating both DNA and RNA from some precious frozen tissue. We have standardized the procedure of DNA separation with GenElute™ Mammalian Genomic DNA Miniprep Kit from...

11 March 2014 878 2 View

How to compare mRNA expression from tissue of different mice?

I usually perform qPCR after stimulating cells, so for every donor I normalized the mRNA expression to the unstimulated sample. But now I have to compare mRNA expression among mice infected with...

30 July 2013 5,220 7 View