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Is there any specific protocol to compare, between 2 groups of patients, if their naive CD4+ T cells are more likely to differentiate (after activation with anti-CD3) into a particular subset ...
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In my experience it worked quite well in mice. Now that I want to see it in humans, it seems that all literature uses an antibody developed by a professor from Freiburg (Germany), and the...
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We are interested in separating both DNA and RNA from some precious frozen tissue. We have standardized the procedure of DNA separation with GenElute™ Mammalian Genomic DNA Miniprep Kit from...
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I usually perform qPCR after stimulating cells, so for every donor I normalized the mRNA expression to the unstimulated sample. But now I have to compare mRNA expression among mice infected with...
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