How can I corroborate the integrity por exosome-derived small RNA?

Hi everyone! I work with Mesenchymal Stem Cells-derived exosomes and I am interested in doing next generation sequencing of exosome-derived small RNAs. After isolation, I obtain a very small...

13 January 2020 7,123 3 View

Transwell Tregs migration assay: fibronectin coating?

I performed this assay many times but without any consistent result. I usually isolate mouse spleen CD4+CD25+ cells, seed 100K non-activated cells on 3um-pore size membranes (24well plate,...

08 November 2019 3,030 1 View

Method for statistical analysis of semiquantitative Western-Blot?

Hello, I need some advice about how to statistically analyze WB data. I am trying to compare the levels of two proteins in mutants relative to wt samples (it's mouse embryonic tissue). I...

10 February 2019 6,052 3 View

How long does it take to an article being cited?

It's depend of knowledge areas or researcher's visibility.

12 February 2018 7,065 7 View

How long does it take to an article being cited?

It's depend of knowledge areas or researcher's visibility.

01 February 2018 4,230 6 View

Where can I buy thermolastic strach (TPS)?

I am looking for a distributer that currently have TPS in stock, and where I could buy a small quantity for research purposes with which I could used to make composites..

12 December 2017 3,118 3 View

In your opinion, does science respond to specific contexts or does science respond to scientometric indicators?

Question about new conditions which researchers are facing, i will appreciate your contribution.

11 December 2017 3,438 3 View

WGS for gram negatives. Better Illumina double read or illumina single read?

I work in gram positives (S. aureus) and lately We have done WGS illumina single read for different clinical and lab strains. The results are really good. Also I had seen publication with...

16 November 2017 4,520 2 View

PLA/cellulose fiber composites, what are the optimal mass concentration for improved mechanical properties?

01 October 2017 336 2 View

What is the maximum amount of SNPs that a strain had to have to be consider isogenic with other?

21 June 2017 2,174 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View