How can I corroborate the integrity por exosome-derived small RNA?

Hi everyone! I work with Mesenchymal Stem Cells-derived exosomes and I am interested in doing next generation sequencing of exosome-derived small RNAs. After isolation, I obtain a very small...

13 January 2020 7,123 3 View

Transwell Tregs migration assay: fibronectin coating?

I performed this assay many times but without any consistent result. I usually isolate mouse spleen CD4+CD25+ cells, seed 100K non-activated cells on 3um-pore size membranes (24well plate,...

08 November 2019 3,030 1 View

Where can I buy thermolastic strach (TPS)?

I am looking for a distributer that currently have TPS in stock, and where I could buy a small quantity for research purposes with which I could used to make composites..

12 December 2017 3,118 3 View

WGS for gram negatives. Better Illumina double read or illumina single read?

I work in gram positives (S. aureus) and lately We have done WGS illumina single read for different clinical and lab strains. The results are really good. Also I had seen publication with...

16 November 2017 4,520 2 View

PLA/cellulose fiber composites, what are the optimal mass concentration for improved mechanical properties?

01 October 2017 336 2 View

What is the maximum amount of SNPs that a strain had to have to be consider isogenic with other?

21 June 2017 2,174 3 View

How liver RNA extract?

Immediately frozen liver with dry ice, I cut a piece of 100mg, I add 1 ml of Trizol reagent, homogenized with homogenizer power and proceed to phase separation, precipitation and RNA wash...

02 November 2016 7,462 5 View

What is the best software for multilevel modelling?

I work with effects of contexts like the place of residence, and use different softwares that fit multilevel models (R, Stata, MLWin, Mplus). Almost any software does this analysis, nowadays (SAS,...

16 November 2013 8,865 8 View

Is it possible that one single compound act as enzyme activator at low concentration but as an enzyme inhibitor when the concentration increase?

Can a single compound increase the enzyme activity at low concentration (below the known inhibition concentration) and when the concentration increase start to inhibit the enzyme? By the way, it...

01 January 1970 7,800 6 View

How to measure outcomes of general education and higher education?

How do you measure the outcomes of general education (up to 12th grade) and higher education (college and above) in a given country? what are some indicators you would suggest I use?

01 January 1970 5,359 5 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

What's the name of important packages for R that generate analysis with mtDNA?

I have mitochondrial DNA sequences to perform analysis of the genetic diversity and population structure of a fish species. What packages can I use on R? I estimate that I need to generate...

01 October 2020 862 5 View

Does Neighbor-Joining (NJ) clustering method suitable to cluster the water quality data?

Dear researchers, I'm using a software called PAST to analyze my multivariate water quality data. For clustering the sampling stations using 14 water quality variables, I found that NJ with...

27 July 2020 2,972 3 View

I want to classification haplotype data by CNN. Can you introduce a suitable network?

hi everybody I converted each word into a vector with one-hot encoding. So that each haplotype has become a two-dimensional matrix. I need CNN to classify them. I appreciate your help. Best regards

25 July 2020 322 2 View

Best phylogenetic analysis for a small data set?

I have a small data set consisting of 16 sequences, 919bp each. I am trying to determine the phylogenetic relationship among the individuals. I am wondering if the neighbor-joining method, maximum...

15 July 2020 3,240 8 View

How to correctly choose population strata and interpret results for the detection of loci under selection from RADseq data using Bayescan?

I am working on the analysis of fungal populations sampled from 12 geographic locations using RADseq data. The dataset contains 7439 polymorphic loci with ~10 snps per RAD locus (75,482 snps...

19 May 2020 643 3 View

Which method most suitable for calculating similarity and genetic distance?

Hi everybody I want to calculate the genetic distance and similarity for ancestral populations. My data is haplotypes. I appreciate your help. Best regards

03 March 2020 8,142 10 View

How To Convert Snp haplotype Data Into 0,1 Matrix? Which software or package is better?

Hi everybody I want to convert HapMap Snp haplotype Data Into 0,1 Matrix so that I can use it in other works. I appreciate your help. Best regards

11 December 2019 414 3 View

How To Convert Snp haplotype Data Into 0,1 Matrix? Which software or package is better?

Hi everybody I want to convert HapMap Snp haplotype Data Into 0,1 Matrix so that I can use it in other works. I appreciate your help. Best regards

02 December 2019 3,312 4 View

How to convert haplotype combinations to data for asssociation study?

Hello all, from 2k SNP dataset I got some 300 haplotype block having 2 to 3 SNPs in each. Can anyone help in conversion of these haplo combinations to data for association study?

10 November 2019 5,980 3 View