Is anyone familiar with stabilization of agar plates with 5-fluorocytosine?

22 September 2014 7,780 1 View

Do you know and use in research any good questionnaire of professional development for preschool/other teachers?

I need a good questionnaire for measuring or valuating (preschool) teacher's professional development - it should be linked with some relevant theories.

16 June 2014 4,419 4 View

What is the best Machine Learning book I can use for implementing text classification?

I am trying to implement a machine learning algorithm that classifies some situations using text messages in log files and in database tables which reside on multiple servers. These messages are...

27 April 2014 6,523 6 View

Methods for solving a particular non-linear system ?

Consider solving the following system for unknowns A1, A2, B, D, G: w = 2 B^2 + D^2; z = A1 B + A2 B + G D; y = 2 A1 A2 + G^2; x = A1^2 + A2^2 + G^2; a2 A1 + a2 A2 + a G = x2 B + x4 B + x3...

27 January 2013 3,834 9 View

Are there any applications of Computable Analysis?

Can you suggest some (real-life) applications of Computable Analysis? http://en.wikipedia.org/wiki/Computable_analysis Why don't we just use classical analysis?

05 July 2012 3,119 4 View

Problem of measuring software entropy (software rot)

What are more recent methods and publications for this ? http://en.wikipedia.org/wiki/Software_rot

22 January 2012 876 1 View

Isometries of the Hilbert Cube

What are the isometries of the Hilbert Cube I^\infty = the set of all sequences (x_i) such that x_i \in [0, 1] endowed with the metric d((x_i), (y_i)) = \sum_{i=0}^\infty 2^{-i} |x_i - y_i| ?

20 November 2011 1,178 3 View

Can someone explain and give some simple examples of applying the Van Kampen theorem for calculating the fundamental group?

I'm interested in a simple explanation of the Van Kampen theorem. What would be a "toy example" for applying it?

18 September 2011 4,693 2 View

Can creativity be effectively automated ? (a note about P versus NP problem)

Once you understand what PvsNP problem is actually all about, you might as well try and solve it. In loose terms, the P vs. NP problem actually seeks an answer to this simply stated...

14 September 2011 2,930 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View