Is it possible that immunocytochemistry and Western blotting gives opposite results?

Hi, is it possible to obtain inverse results by immunocytochemistry and Western blotting. Experimental series with low fluorescence in nuclei show strong band in Western blot and oppositely...

26 May 2016 8,737 4 View

Is anyone familiar with stabilization of agar plates with 5-fluorocytosine?

22 September 2014 7,780 1 View

Do you know and use in research any good questionnaire of professional development for preschool/other teachers?

I need a good questionnaire for measuring or valuating (preschool) teacher's professional development - it should be linked with some relevant theories.

16 June 2014 4,419 4 View

What is the best Machine Learning book I can use for implementing text classification?

I am trying to implement a machine learning algorithm that classifies some situations using text messages in log files and in database tables which reside on multiple servers. These messages are...

27 April 2014 6,523 6 View

Methods for solving a particular non-linear system ?

Consider solving the following system for unknowns A1, A2, B, D, G: w = 2 B^2 + D^2; z = A1 B + A2 B + G D; y = 2 A1 A2 + G^2; x = A1^2 + A2^2 + G^2; a2 A1 + a2 A2 + a G = x2 B + x4 B + x3...

27 January 2013 3,834 9 View

Are there any applications of Computable Analysis?

Can you suggest some (real-life) applications of Computable Analysis? http://en.wikipedia.org/wiki/Computable_analysis Why don't we just use classical analysis?

05 July 2012 3,119 4 View

Problem of measuring software entropy (software rot)

What are more recent methods and publications for this ? http://en.wikipedia.org/wiki/Software_rot

22 January 2012 876 1 View

Isometries of the Hilbert Cube

What are the isometries of the Hilbert Cube I^\infty = the set of all sequences (x_i) such that x_i \in [0, 1] endowed with the metric d((x_i), (y_i)) = \sum_{i=0}^\infty 2^{-i} |x_i - y_i| ?

20 November 2011 1,178 3 View

Can someone explain and give some simple examples of applying the Van Kampen theorem for calculating the fundamental group?

I'm interested in a simple explanation of the Van Kampen theorem. What would be a "toy example" for applying it?

18 September 2011 4,693 2 View

Can creativity be effectively automated ? (a note about P versus NP problem)

Once you understand what PvsNP problem is actually all about, you might as well try and solve it. In loose terms, the P vs. NP problem actually seeks an answer to this simply stated...

14 September 2011 2,930 1 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

No title

Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

What are some common oxidants used to oxidize iron sulfur clusters in proteins for techniques like EPR?

I have used potassium ferricyanide (which is likely the most common) extensively in the past, but I am curious to know if there are other oxidants that work well. I need to generate the oxidized...

29 January 2021 9,803 4 View

Papain Enzyme Assay...?

Hi all, I would like to know how can I estimate the activity of the enzyme, papain, which is used in reactions as crude latex extract of papaya. Please share some procedures if anybody having...

26 January 2021 569 4 View

Where can I find plants pyr-GC-MS data?

25 January 2021 5,403 2 View

Recombinant restriction enzyme: how strong should the corresponding methyltransferase expression level be?

Hello. We are planning expression of a restriction enzyme in E. coli, and wondering how much of the corresponding methyltransferase is needed to be expressed in order to protect the host genome...

23 January 2021 2,096 4 View